Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency.
Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency.
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DOI：
10.1002/cm.21010
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发表时间：
2012-03
期刊：
影响因子：
2.9
通讯作者：
Smith, Elizabeth F.
中科院分区：
文献类型：
--
作者：
Goduti, Daniel J.;Smith, Elizabeth F.
大量研究表明，与中央微管对相关的七个突起中的每一个都在调节真核纤毛/鞭毛运动中发挥着独特的作用。缺乏特异突起的突变体具有不同的运动表型。例如，衣单胞菌pf6突变体缺乏C1a投射，具有抽搐的、不跳动的鞭毛。C1a投射是一个蛋白质复合物，包括PF6、C1a-86、C1a-34、C1a-32、C1a-18和钙调蛋白。为了确定PF6的功能域，并潜在地将功能分配给特定的C1a组件，我们生成了PF6基因的缺失构建体，并测试了它们在突变PF6细胞转化时组装和恢复运动的能力。我们的研究结果表明，PF6的羧基末端附近的结构域对投射的运动和/或组装至关重要。PF6的氨基末端一半不需要用于C1a组装；然而，该区域对C1a-34、C1a-32和C1a-18亚复合物和野生型心跳频率的稳定性很重要。对缺乏PF6和外动力蛋白臂氨基末端的双突变体的分析表明，C1a可能在调节内动力蛋白臂和外动力蛋白臂活性中发挥作用。
Numerous studies have indicated that each of the seven projections associated with the central pair of microtubules plays a distinct role in regulating eukaryotic ciliary / flagellar motility. Mutants which lack specific projections have distinct motility phenotypes. For example, Chlamydomonas pf6 mutants lack the C1a projection and have twitchy, non-beating flagella. The C1a projection is a complex of proteins including PF6, C1a-86, C1a-34, C1a-32, C1a-18 and calmodulin. To define functional domains within PF6 and to potentially assign functions to specific C1a components, we generated deletion constructs of the PF6 gene and tested for their ability to assemble and rescue motility upon transformation of mutant pf6 cells. Our results demonstrate that domains near the carboxyl-terminus of PF6 are essential for motility and/or assembly of the projection. The amino terminal half of PF6 is not required for C1a assembly; however, this region is important for stability of the C1a-34, C1a-32, and C1a-18 sub-complex and wild-type beat frequency. Analysis of double mutants lacking the amino terminus of PF6 and outer dynein arms reveal that C1a may play a role in modulating both inner and outer dynein arm activity.
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影响因子：
7.2
作者：
Molnar, Attila;Bassett, Andrew;Baulcombe, David
通讯作者：
Baulcombe, David
影响因子：
5.3
作者：
Lee, Lance;Campagna, Dean R.;Fleming, Mark D.
通讯作者：
Fleming, Mark D.
衣原体插入突变体的表征，该突变体破坏了鞭毛中心对微管相关结构。
影响因子：
7.8
作者：
Mitchell, D R;Sale, W S
通讯作者：
Sale, W S
DOI：
10.1083/jcb.98.1.229
发表时间：
1984-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dutcher SK;Huang B;Luck DJ
通讯作者：
Luck DJ
DOI：
10.1083/jcb.200912009
发表时间：
2010-05-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
DiPetrillo CG;Smith EF
通讯作者：
Smith EF