Immunization with SARS-CoV S DNA vaccine generates memory CD4+ and CD8+ T cell immune responses.
Immunization with SARS-CoV S DNA vaccine generates memory CD4+ and CD8+ T cell immune responses.
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DOI：
10.1016/j.vaccine.2006.03.058
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发表时间：
2006-06-05
期刊：
影响因子：
5.5
通讯作者：
Wu CY
中科院分区：
文献类型：
--
作者：
Huang J;Ma R;Wu CY
严重急性呼吸系统综合症（SARS）的有效疫苗可能需要产生和维持体液和细胞免疫反应。据报道，经人自然感染和动物接种SARS-CoV疫苗后，产生抗体并持续较长时间。在本研究中，用SARS- cov S DNA疫苗免疫小鼠，并使用三种不同的方法（ELISA， ELISPOT和FACS）在体外用重叠整个SARS刺突蛋白的肽池刺激细胞时评估免疫反应。结果表明，SARS-CoV S DNA疫苗原代免疫可诱导CD4+和CD8+ T细胞反应。用同样的疫苗增强CD4+和CD8+ T细胞在淋巴和非淋巴器官的反应，并持续超过两个月。SARS-CoV s特异性CD4+和CD8+ T细胞为CD62L -，这是记忆细胞的标志，−30 ~ 50%的细胞表达IL-7Rα (CD127)，这是效应细胞发育为记忆细胞的能力的标志。此外，DNA疫苗免疫可引起高水平的抗体产生。综上所述，这些数据表明，SARS-CoV S DNA疫苗免疫可产生抗原特异性体液和细胞免疫反应，可能有助于长期保护。
An effective vaccine for severe acute respiratory syndrome (SARS) will probably require the generation and maintenance of both humoral and cellular immune responses. It has been reported that after natural infection in humans and immunization in animals with SARS-CoV vaccine, antibody is produced and persistent for a long period of time. In the present study, mice were immunized i.m. with SARS-CoV S DNA vaccine, and three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses when the cells were stimulated in vitro with a pool of peptides overlapping entire SARS spike protein. The results show that prime-immunization with SARS-CoV S DNA vaccine can induce both CD4+ and CD8+ T cell responses. Boosting with the same vaccine enhances CD4+ and CD8+ T cell responses in both lymphoid and nonlymphoid organs and were persistent over two months. The SARS-CoV S-specific CD4+ and CD8+ T cells were CD62L−, a marker for memory cells, and −30 to 50% of the cells expressed IL-7Rα (CD127), a marker for the capacity of effector cells to develop into memory cells. In addition, immunization with the DNA vaccine elicited high levels of antibody production. Taken together, these data demonstrate that immunization with SARS-CoV S DNA vaccine can generate antigen-specific humoral and cellular immune responses that may contribute to long-term protection.
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影响因子：
9.4
作者：
Woo, PCY;Lau, SKP;Yuen, K
通讯作者：
Yuen, K
通过用N，M和E基因诱导DNA疫苗对小鼠SARS-COV的DNA疫苗接种。
DOI：
10.1016/j.bbrc.2005.01.048
发表时间：
2005-03-25
影响因子：
3.1
作者：
Jin H;Xiao C;Chen Z;Kang Y;Ma Y;Zhu K;Xie Q;Tu Y;Yu Y;Wang B
通讯作者：
Wang B
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
影响因子：
30.5
作者：
Wu, CY;Kirman, JR;Seder, RA
通讯作者：
Seder, RA
影响因子：
82.9
作者：
Traggiai E;Becker S;Subbarao K;Kolesnikova L;Uematsu Y;Gismondo MR;Murphy BR;Rappuoli R;Lanzavecchia A
通讯作者：
Lanzavecchia A