Mechanisms and implications of air pollution particle associations with chemokines.
Mechanisms and implications of air pollution particle associations with chemokines.
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DOI：
10.1016/j.taap.2008.08.001
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发表时间：
2008-11-01
影响因子：
3.8
通讯作者：
Seagrave, JeanClare
中科院分区：
文献类型：
--
作者：
Seagrave, JeanClare
吸入空气污染物颗粒引起的炎症被认为是暴露在空气污染中对健康造成不良影响的一种机制。炎症反应与多种促炎细胞因子和趋化因子的上调有关。我们以前已经证明，柴油废气颗粒物(DEP)是许多城市空气污染颗粒物的重要组成部分，它结合并浓缩IL-8，这是一种重要的人类中性粒细胞趋化因子，并且该趋化因子仍然具有生物活性。在这份报告中，我们研究了这种联系的可能机制以及对趋化因子清除的影响。这种结合似乎是带负电的颗粒和带正电的趋化因子分子之间离子相互作用的结果，可能与嵌入颗粒中的小孔相结合。这种关联并不局限于柴油废气颗粒和IL-8：其他几种类型的颗粒也吸附趋化因子，几种其他细胞因子也吸附在柴油颗粒上。然而，不同类型的颗粒和不同的细胞因子的有效性存在很大的差异。最后，在异氟烷麻醉下，雄性Fisher 344大鼠气管内注入趋化因子，或联合柴油尾气或二氧化硅颗粒。与不结合趋化因子的二氧化硅颗粒相反，柴油废气颗粒与趋化因子结合在一起，延长了趋化因子的保留时间。
Inflammation induced by inhalation of air pollutant particles has been implicated as a mechanism for the adverse health effects associated with exposure to air pollution. The inflammatory response is associated with upregulation of various pro-inflammatory cytokines and chemokines. We have previously shown that diesel exhaust particles (DEP), a significant constituent of air pollution particulate matter in many urban areas, bind and concentrate IL-8, an important human neutrophil-attracting chemokine, and that the chemokine remains biologically active. In this report, we examine possible mechanisms of this association and the effects on clearance of the chemokine. The binding appears to be the result of ionic interactions between negatively charged particles and positively charged chemokine molecules, possibly combined with intercalation into small pores in the particles. The association is not limited to diesel exhaust particles and IL-8: several other particle types also adsorb the chemokine and several other cytokines are adsorbed onto the diesel particles. However, there are wide ranges in the effectiveness of various particle types and various cytokines. Finally, male Fisher 344 rats were intratracheally instilled with chemokine alone or combined with diesel exhaust or silica particles under isofluorane anesthesia. In contrast to silica particles, which do not bind the chemokine, the presence of diesel exhaust particles, which bind the chemokine, prolonged the retention of the chemokine.
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DOI：
10.1073/pnas.92.11.5042
发表时间：
1995-05-23
影响因子：
11.1
作者：
MCKEITHAN, TW
通讯作者：
MCKEITHAN, TW
影响因子：
4.5
作者：
Ichinose, T;Takano, H;Sagai, M
通讯作者：
Sagai, M
影响因子：
4.3
作者：
Ito, T;Ikeda, M;Tomita, T
通讯作者：
Tomita, T
DOI：
10.1165/ajrcmb.24.4.4085
发表时间：
2001-04-01
影响因子：
6.4
作者：
Kawasaki, S;Takizawa, H;Ohtoshi, T
通讯作者：
Ohtoshi, T
影响因子：
5.2
作者：
McDonald, JD;Barr, EB;White, RK
通讯作者：
White, RK