AGC protein kinase AGC1-4 mediates seed size in Arabidopsis
AGC protein kinase AGC1-4 mediates seed size in Arabidopsis
复制标题
AGC 蛋白激酶 AGC1-4 介导拟南芥种子大小
DOI：
10.1007/s00299-020-02533-z
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发表时间：
2020-03
影响因子：
6.2
通讯作者：
Xian Sheng Zhang
中科院分区：
文献类型：
--
作者：
Yu Ying Zhang;Wang Jinsong Yao;Fang Wang;Ying Hua Su;Da Jian Zhang;Sheng Wu Hu;Xian Sheng Zhang
AGC 1 -4激酶通过介导拟南芥的细胞增殖和胚胎发育在种子调控中起着至关重要的作用。种子大小是影响植物种子最终产量的重要因素。然而，确定最终种子大小的分子机制仍然需要研究。在这里，我们确定了一个新的AGC蛋白激酶AGC 1 -4，它编码的丝氨酸-苏氨酸激酶，属于AGC VIIIa亚家族。agc 1 -4突变体的种子显著大于野生型。AGC 1 -4基因的过表达降低了种子大小。AGC 1 -4种子大小的调节依赖于胚胎细胞数量。为了进一步确定AGC 1 -4在种子大小中的功能，我们使用无标记定量磷酸蛋白质组学分析AGC 1 -4磷蛋白，该定量磷酸蛋白质组学使用RNA测序（RNA-seq）与agc 1 -4的转录组偶联。RNA-seq分析显示了1611个差异表达基因（DEG），涵盖了细胞周期和胚胎发育等广泛的功能。通过磷酸化蛋白质组学分析，检测到262个独特的磷酸化蛋白。差异磷酸化蛋白质参与细胞周期和胚胎后发育。RNA-seq和磷酸化蛋白质组学结果的叠加表明，AGC 1 -4是通过介导细胞增殖和胚胎发育影响种子大小的重要因素。本研究结果为丝氨酸-苏氨酸激酶AGC 1 -4介导拟南芥种子大小提供了新的数据。
AGC1-4 kinase plays a crucial role in the regulation of seeds by mediating cell proliferation and embryo development in Arabidopsis. Seed size is a crucial factor to influence final seed yield in plants. However, the molecular mechanisms that set final seed size still need to be investigated. Here, we identified a novel AGC protein kinase AGC1-4, which encodes a serine–threonine kinase, belongs to the AGC VIIIa subfamily. The seeds of agc1-4 mutant were significantly larger than that in the wild type. Overexpression of the AGC1-4 gene reduced seed size. Regulation of AGC1-4 seed size is dependent on embryonic cell number. To further determine AGC1-4 functions in seed size, we analyzed AGC1-4 phosphoproteins using label-free quantitative phosphoproteomics coupled to the transcriptome of agc1-4 using RNA sequencing (RNA-seq). The RNA-seq analysis showed 1611 differentially expressed genes (DEGs), which cover a wide range of functions, such as cell cycle and embryo development. The 262 unique phosphoproteins were detected by phosphoproteomics analysis. The differentially phosphorylated proteins were involved in cell cycle and post-embryo development. Overlay of the RNA-seq and phosphoproteomics results demonstrated AGC1-4 as an important factor that influences seed size by mediating cell proliferation and embryo development. The results in this study provide novel data on the serine–threonine kinase AGC1-4 mediating seed size in Arabidopsis.
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影响因子：
4.6
作者：
René Benjamins;Ab Quint;D. Weijers;P. Hooykaas;R. Offringa
通讯作者：
René Benjamins;Ab Quint;D. Weijers;P. Hooykaas;R. Offringa
影响因子：
11.6
作者：
Fiume, Elisa;Fletcher, Jennifer C.
通讯作者：
Fletcher, Jennifer C.
影响因子：
11.4
作者：
Xiao-Ran Zhang;Zhixiang Qin;Xiao Zhang;Yuxin Hu
通讯作者：
Xiao-Ran Zhang;Zhixiang Qin;Xiao Zhang;Yuxin Hu
影响因子：
7.2
作者：
Lahmy, S;Guilleminot, J;Devic, M
通讯作者：
Devic, M
影响因子：
11.6
作者：
Garcia, D;Fitz Gerald, JN;Berger, F
通讯作者：
Berger, F