Multifunctional nanoplatforms for fluorescence imaging and photodynamic therapy developed by post-loading photosensitizer and fluorophore to polyacrylamide nanoparticles.
Multifunctional nanoplatforms for fluorescence imaging and photodynamic therapy developed by post-loading photosensitizer and fluorophore to polyacrylamide nanoparticles.
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DOI：
10.1016/j.nano.2011.11.011
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发表时间：
2012-08
影响因子：
5.4
通讯作者：
Pandey, Ravindra K.
中科院分区：
文献类型：
--
作者：
Gupta, Anurag;Wang, Shouyan;Pera, Paula;Rao, K. V. R.;Patel, Nayan;Ohulchanskyy, Tymish Y.;Missert, Joseph;Morgan, Janet;Koo-Lee, Yong-Eun;Kopelman, Raoul;Pandey, Ravindra K.
我们报告了一种新的后加载方法，用于构建多功能生物可降解聚丙烯酰胺（PAA）纳米平台，用于肿瘤成像（荧光）和光动力学治疗（PDT）。该方法提供了以所需浓度后加载成像剂和治疗剂的机会。在PAA纳米颗粒中，含有光敏剂HPPH [3-（1 '-己氧基乙基）焦脱镁叶绿酸-a]和花青染料的制剂以2：1的比例最小化了由于纳米颗粒内的能量迁移和/或HPPH和花青染料之间的Förster（荧光）共振能量转移（FRET）引起的HPPH电子激发能量的不期望的淬灭。证明了纳米构建体制剂的优异的肿瘤成像（NIR荧光）和光治疗功效。在类似的处理参数下，在1%吐温80/5%葡萄糖水溶液制剂中的HPPH比以2：1的比例含有HPPH和花青染料的纳米构建体更不有效。这是第一个显示后加载方法在开发用于肿瘤成像和治疗的纳米结构中的效用的例子。
We report a novel post-loading approach for constructing a multifunctional biodegradable polyacrylamide (PAA) nanoplatform for tumor-imaging (fluorescence) and photodynamic therapy (PDT). This approach provides an opportunity to post-load the imaging and therapeutic agents at desired concentrations. Among the PAA nanoparticles, a formulation containing the photosensitizer, HPPH [3-(1’-hexyloxyethyl)pyropheophorbide-a], and the cyanine dye in a ratio of 2:1 minimized the undesirable quenching of the HPPH electronic excitation energy due to energy migration within the nanoparticles and/or Förster (fluorescence) resonance energy transfer (FRET) between HPPH and cyanine dye. An excellent tumor-imaging (NIR fluorescence) and phototherapeutic efficacy of the nanoconstruct formulation is demonstrated. Under similar treatment parameters the HPPH in 1% Tween 80/5% aqueous dextrose formulation was less effective than the nanoconstruct containing HPPH and cyanine dye in a ratio of 2 to 1. This is the first example showing the utility of the post-loading approach in developing a nanoconstructs for tumor-imaging and therapy.
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影响因子：
10.8
作者：
Burns AA;Vider J;Ow H;Herz E;Penate-Medina O;Baumgart M;Larson SM;Wiesner U;Bradbury M
通讯作者：
Bradbury M
影响因子：
2.7
作者：
Kopelman, R;Koo, YEL;Ross, BD
通讯作者：
Ross, BD
影响因子：
10.8
作者：
Nasongkla, Norased;Bey, Erik;Gao, Jinming
通讯作者：
Gao, Jinming
影响因子：
11.5
作者：
Chen, B;Pogue, BW;Hasan, T
通讯作者：
Hasan, T
影响因子：
17.1
作者：
Meng H;Xue M;Xia T;Ji Z;Tarn DY;Zink JI;Nel AE
通讯作者：
Nel AE