Drosophila Ge-1 promotes P body formation and oskar mRNA localization.
Drosophila Ge-1 promotes P body formation and oskar mRNA localization.
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DOI：
10.1371/journal.pone.0020612
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ephrussi A
中科院分区：
文献类型：
--
作者：
Fan SJ;Marchand V;Ephrussi A
mRNA定位与翻译控制相结合是一种广泛而保守的策略，它允许真核细胞内蛋白质的本地化生产。在果蝇中，oskar (osk) mRNA在卵母细胞后极的定位和翻译对于胚胎的正确模式至关重要。一些P小体成分参与了osk mRNA的定位和翻译抑制，这表明P小体和osk RNPs之间存在联系。在培养的哺乳动物细胞中，P体的形成需要Ge-1蛋白。结合遗传、生化和免疫组织化学方法，我们发现，在体内，果蝇Ge-1 （dGe-1）是一个重要的基因，编码P体成分，促进生殖系中这些结构的形成。dGe-1部分与osk mRNA共定位，是osk RNP完整性所必需的。我们的分析表明，尽管在正常情况下，dGe-1的功能对osk mRNA的定位不是必需的，但当定位机制的其他组成部分，如staufen、果蝇脱帽蛋白1和barentsz受到限制时，它就变得至关重要。我们的研究结果表明，dGe-1在优化果蝇胚胎图案化所需的osk mRNA定位过程中发挥重要作用。
mRNA localization coupled with translational control is a widespread and conserved strategy that allows the localized production of proteins within eukaryotic cells. In Drosophila, oskar (osk) mRNA localization and translation at the posterior pole of the oocyte are essential for proper patterning of the embryo. Several P body components are involved in osk mRNA localization and translational repression, suggesting a link between P bodies and osk RNPs. In cultured mammalian cells, Ge-1 protein is required for P body formation. Combining genetic, biochemical and immunohistochemical approaches, we show that, in vivo, Drosophila Ge-1 (dGe-1) is an essential gene encoding a P body component that promotes formation of these structures in the germline. dGe-1 partially colocalizes with osk mRNA and is required for osk RNP integrity. Our analysis reveals that although under normal conditions dGe-1 function is not essential for osk mRNA localization, it becomes critical when other components of the localization machinery, such as staufen, Drosophila decapping protein 1 and barentsz are limiting. Our findings suggest an important role of dGe-1 in optimization of the osk mRNA localization process required for patterning the Drosophila embryo.
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影响因子：
4
作者：
Reijns MA;Alexander RD;Spiller MP;Beggs JD
通讯作者：
Beggs JD
影响因子：
64.5
作者：
LEHMANN, R;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C
影响因子：
56.9
作者：
Brendza, RP;Serbus, LR;Saxton, WM
通讯作者：
Saxton, WM
影响因子：
10.5
作者：
Besse, Florence;de Quinto, Sonia Lopez;Ephrussi, Anne
通讯作者：
Ephrussi, Anne
DOI：
10.1083/jcb.200504039
发表时间：
2005-09-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ferraiuolo MA;Basak S;Dostie J;Murray EL;Schoenberg DR;Sonenberg N
通讯作者：
Sonenberg N