Regulating Activity of Polysaccharides from Portulaca oleracea L. on Dendritic Cells of Mice Immunized against Foot-and-Mouth Disease
Regulating Activity of Polysaccharides from Portulaca oleracea L. on Dendritic Cells of Mice Immunized against Foot-and-Mouth Disease
复制标题
马齿苋多糖对口蹄疫免疫小鼠树突状细胞活性的调节
DOI：
10.29261/pakvetj/2018.126
复制
发表时间：
2019
影响因子：
2.3
通讯作者：
Lingyu Wu
中科院分区：
文献类型：
--
作者：
Rui Zhao;Guiyan Jia;Yongzhong Yu;Bocui Song;Chao Hu;Lingyu Wu
已接收: 修订: 已接受: 在线发布: 2018年2月16日 2018年6月18日 2018年6月19日 2019年12月26日 先前的研究表明马齿苋多糖（POL-P3b）具有佐剂活性。在免疫反应过程中，树突状细胞（DC）发挥着重要作用。鉴于此，通过口服POL-P3b作为口蹄疫（FMD）疫苗的佐剂来研究DC的成熟和增强功能。进一步确定了所涉及的机制。我们发现，给予不同浓度的 POL-P3b 通过增加共刺激和 MHC-II 表达来诱导 DC 成熟。此外，POL-P3b提高了T淋巴细胞的刺激活性，且高剂量更明显。此外，POL-P3b 使细胞因子分泌偏向 Th1 途径。有趣的是，DC+POL-P3b组线粒体膜电位含量显着升高。为了阐明其机制，进一步研究了 POL-P3b 对 TLR4 信号通路的影响。我们的结果表明，POL-P3b 处理增加了 TLR4 的表达，并减少了 caspase-3、-9 和 -8 的表达。总体而言，实验结果表明，POL-P3b可以通过诱导DC成熟并增强其功能，作为FMD疫苗的有效佐剂，其机制与调节TLR4信号通路有关。 ©2018 PVJ。版权所有
Received: Revised: Accepted: Published online: February 16, 2018 June 18, 2018 June 19, 2018 December 26, 2019 Previous study has showed that portulaca oleracea L. polysaccharide (POL-P3b), possess adjuvant activity. In the process of immune response, dendritic cells (DC) play a significant role. In view of this, DC maturation and enhancing function were investigated by oral administration with POL-P3b, as an adjuvant for foot-andmouth disease (FMD) vaccine. The mechanisms involved were further identified. We found that administration with POL-P3b at different concentrations induced DC maturation by increasing co-stimulatory and MHC-II expression. Furthermore, POL-P3b improved stimulus activity of T lymphocyte, and the high dose was more pronounced. In addition, POL-P3b polarized cytokine secretion toward the Th1 pathway. Interestingly, the content of mitochondrial membrane potential was significantly increased in DC+POL-P3b group. To clarify the mechanisms, the effects of POL-P3b on TLR4 signaling pathways were further investigated. Our results showed that POL-P3b treatment increased TLR4 expression and decreased the expression of caspase-3, -9 and -8. On the whole, the experimental results suggested that POL-P3b could be used as an effective adjuvant for FMD vaccine by inducing maturation and enhancing function of DCs, and the mechanism was related to regulating TLR4 signaling pathway. ©2018 PVJ. All rights reserved
登录
查看更多内容
影响因子：
7.3
作者：
Salazar F;Ghaemmaghami AM
通讯作者：
Ghaemmaghami AM
影响因子：
64.8
作者：
Park, Daeho;Han, Claudia Z.;Elliott, Michael R.;Kinchen, Jason M.;Trampont, Paul C.;Das, Soumita;Collins, Sheila;Lysiak, Jeffrey J.;Hoehn, Kyle L.;Ravichandran, Kodi S.
通讯作者：
Ravichandran, Kodi S.
口服人参茎叶皂苷增强口蹄疫疫苗免疫反应
影响因子：
3.3
作者：
Li, Renjun;Ma, Yanfen;Hu, Songhua
通讯作者：
Hu, Songhua
DOI：
10.1134/s1990750817030076
发表时间：
2017-08
期刊：
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry
影响因子：
--
作者：
I. Makarenkova;N. Akhmatova;S. Ermakova;N. Besednova
通讯作者：
I. Makarenkova;N. Akhmatova;S. Ermakova;N. Besednova
DOI：
--
发表时间：
2004
期刊：
Communications in agricultural and applied biological sciences
影响因子：
--
作者：
J. Menéndez;F. Bastida
通讯作者：
J. Menéndez;F. Bastida