Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas
Expression Characterization of Stress Genes Under High and Low Temperature Stresses in the Pacific Oyster, Crassostrea gigas
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巨牡蛎高低温胁迫下胁迫基因的表达特征
DOI：
10.1007/s10126-015-9678-0
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发表时间：
2016-01
影响因子：
3
通讯作者：
Zhang Guofan
中科院分区：
文献类型：
--
作者：
Zhu Qihui;Zhang Linlin;Li Li;Que Huayong;Zhang Guofan
太平洋牡蛎（Crassostrea gigas）是潮间带特有的固着生物，它生活在地球上最具物理压力的环境之一。由于高度暴露于陆地条件，牡蛎必须耐受温度范围的广泛波动。然而，牡蛎对温度胁迫的细胞和分子反应尚未完全表征。在这里，我们分析了牡蛎在高温和低温下的转录组数据。我们还确定了30多个关键的温度胁迫响应候选基因，编码应激蛋白，如热休克蛋白和凋亡相关蛋白。通过实时荧光定量PCR检测这些基因在短期冷、热环境（5 °C和35 °C）和长期冷环境（5 °C）下的表达特征。这些基因大多在热胁迫24 h后的恢复期达到表达高峰，在长期冷胁迫下，这些基因在第3 d左右表达量显著增加，而对短期冷胁迫反应不大。另外，在恢复2 d后的第二次热胁迫中，牡蛎表现出这些基因的温和表达和较低的死亡率，这表明牡蛎对热胁迫的反应存在可塑性。我们证实了牡蛎的稳态弹性和抗凋亡可能是温度胁迫反应的关键中心。此外，我们分析了11个不同物种的胁迫基因家族，发现胁迫基因的谱系特异性扩展可能与适应性进化有关。这些结果表明，可塑性和进化在牡蛎的应激反应适应中起着重要作用。
As a characteristic sessile inhabitant of the intertidal zone, the Pacific oyster Crassostrea gigas occupies one of the most physically stressful environments on earth. With high exposure to terrestrial conditions, oysters must tolerate broad fluctuations in temperature range. However, oysters' cellular and molecular responses to temperature stresses have not been fully characterized. Here, we analyzed oyster transcriptome data under high and low temperatures. We also identified over 30 key temperature stress-responsive candidate genes, which encoded stress proteins such as heat shock proteins and apoptosis-associated proteins. The expression characterization of these genes under short-term cold and hot environments (5 and 35 °C) and long-term cold environments (5 °C) was detected by quantitative real-time PCR. Most of these genes reached expression peaks during the recovery stage after 24 h of heat stress, and these genes were greatly induced around day 3 in long-term cold stress while responded little to short-term cold stress. In addition, in the second heat stress after 2 days of recovery, oysters showed milder expression in these genes and a lower mortality rate, which indicated the existence of plasticity in the oyster's response to heat stress. We confirmed that homeostatic flexibility and anti-apoptosis might be crucial centers of temperature stress responses in oysters. Furthermore, we analyzed stress gene families in 11 different species and found that the linage-specific expansion of stress genes might be implicated in adaptive evolution. These results indicated that both plasticity and evolution played an important role in the stress response adaptation of oysters.
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影响因子：
1.3
作者：
Guo, Ximing;Lio, Xiao;Wang, Haiyan;Zhang, Guofan
通讯作者：
Zhang, Guofan
影响因子：
14.9
作者：
Cameron RA;Samanta M;Yuan A;He D;Davidson E
通讯作者：
Davidson E
影响因子：
2.7
作者：
CRAIG, EA;INGOLIA, TD;MANSEAU, LJ
通讯作者：
MANSEAU, LJ
影响因子：
4.5
作者：
S. A. Jackson;K. Uhlinger;J. Clegg
通讯作者：
S. A. Jackson;K. Uhlinger;J. Clegg
DOI：
--
发表时间：
1996-08
期刊：
The Journal of experimental biology
影响因子：
--
作者：
J. Stillman;G. Somero
通讯作者：
J. Stillman;G. Somero