Soy compared with milk protein in a Western diet changes fecal microbiota and decreases hepatic steatosis in obese OLETF rats.
Soy compared with milk protein in a Western diet changes fecal microbiota and decreases hepatic steatosis in obese OLETF rats.
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DOI：
10.1016/j.jnutbio.2017.05.004
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Rector RS
中科院分区：
文献类型：
--
作者：
Panasevich MR;Schuster CM;Phillips KE;Meers GM;Chintapalli SV;Wankhade UD;Shankar K;Butteiger DN;Krul ES;Thyfault JP;Rector RS
大豆蛋白可有效预防肝脂肪变性，但其机制尚不清楚。我们检验了以下假设，即大豆与乳制品蛋白质为主的饮食会改变大冢Long-Evans德岛脂肪（OLETF）大鼠的微生物群并减轻肝脏脂肪变性。将雄性OLETF大鼠随机分配至含有乳蛋白分离物（MPI）、大豆蛋白分离物（SPI）或50：50 MPI/SPI（MS）的“西方”饮食（n=9-10/组; 21%kcal蛋白），持续16周。与MS相比，SPI使脂肪量和脂肪百分比减少约10%（P<0.05），但与MPI相比则不然。血清TBAR、总胆固醇和LDL-胆固醇浓度较低与MPI和MS相比，SPI组的肝脏脂肪变性较低（P<0.05）。与MPI或MS相比，SPI中的蛋白质水平降低（P<0.05）。SPI组与MPI组相比，肝脏二酰甘油水平显著降低（P<0.05），而三酰甘油水平无显著变化，这与肝脏新生脂肪生成较低有关（ACC、FAS和SCD-1蛋白含量以及肝脏16：1 n-7和18：1 n-7 PUFA浓度）与MPI、MS比较，P<0.05;但是，在此情况下，与SPI和MS相比，MPI显示肝线粒体功能升高。粪便细菌16 S rRNA分析显示SPI摄入引起肝线粒体功能增加在乳酸杆菌中的降低（P<0.05）以及在布劳特氏菌和毛螺菌科中的降低（P<0.05），表明SPI大鼠粪便次级胆汁酸的降低。SPI和MS组肝脏Fxr、Fgfr 4、Hnf 4a、HmgCoA还原酶和合成酶mRNA表达均显著高于MPI组（P<0.05）。总体而言，饮食SPI相比MPI减少肝脂肪变性和二酰基甘油，改变微生物群种群，并改变胆汁酸信号和胆固醇稳态的啮齿动物模型的肥胖。
Soy protein is effective at preventing hepatic steatosis; however, the mechanisms are poorly understood. We tested the hypothesis that soy versus dairy protein-based diet would alter microbiota and attenuate hepatic steatosis in hyperphagic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Male OLETF rats were randomized to “Western” diets containing milk protein isolate (MPI), soy protein isolate (SPI), or 50:50 MPI/SPI (MS) (n=9–10/group; 21% kcal protein) for 16 weeks. SPI attenuated (P<0.05) fat mass and percent fat by ~10% compared with MS, but not compared with MPI. Serum TBAR and total and LDL-cholesterol concentrations were lower (P<0.05) with dietary SPI versus MPI and MS. Histological hepatic steatosis was lower (P<0.05) in SPI compared with MPI or MS. Lipidomic analyses revealed reductions (P<0.05) in hepatic diacylglycerols but not triacylglycerols in SPI compared with MPI, which was associated with lower hepatic de novo lipogenesis (ACC, FAS, and SCD-1 protein content, and hepatic 16:1 n-7 and 18:1 n-7 PUFA concentrations) (P<0.05) compared with MPI and MS; however, MPI displayed elevated hepatic mitochondrial function compared with SPI and MS. Fecal bacterial 16S rRNA analysis revealed SPI-intake elicited increases (P<0.05) in Lactobacillus and decreases (P<0.05) in Blautia and Lachnospiraceae suggesting decreases in fecal secondary bile acids in SPI rats. SPI and MS exhibited greater (P<0.05) hepatic Fxr, Fgfr4, Hnf4a, HmgCoA reductase and synthase mRNA expression compared with MPI. Overall, dietary SPI compared with MPI decreased hepatic steatosis and diacylglycerols, changed microbiota populations, and altered bile acid signaling and cholesterol homeostasis in a rodent model of obesity.
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影响因子：
64.5
作者：
Cao H;Gerhold K;Mayers JR;Wiest MM;Watkins SM;Hotamisligil GS
通讯作者：
Hotamisligil GS
影响因子：
16.2
作者：
Azadbakht, Leila;Atabak, Shahnaz;Esmaillzadeh, Ahmad
通讯作者：
Esmaillzadeh, Ahmad
影响因子：
4.1
作者：
Fletcher JA;Meers GM;Linden MA;Kearney ML;Morris EM;Thyfault JP;Rector RS
通讯作者：
Rector RS
影响因子：
2.2
作者：
Cain, J.;Banz, W. J.;Davis, J. E.
通讯作者：
Davis, J. E.
影响因子：
4.4
作者：
Kozich, James J.;Westcott, Sarah L.;Schloss, Patrick D.
通讯作者：
Schloss, Patrick D.