ZnO nanoparticles affect intestinal function in an in vitro model.
ZnO nanoparticles affect intestinal function in an in vitro model.
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DOI：
10.1039/c7fo02038d
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发表时间：
2018-03-01
期刊：
影响因子：
6.1
通讯作者：
Mahler GJ
中科院分区：
文献类型：
--
作者：
Moreno-Olivas F;Tako E;Mahler GJ
氧化锌纳米颗粒（ZnO NP）可能存在于食品包装中，这将使消费者面临NP摄入的风险。关于食品包装中存在的ZnO NP的量以及ZnO暴露对肠道功能的影响的信息很少。为了估计生理相关的ZnO暴露，用电感耦合等离子体质谱法（ICP-MS）分析了天然低锌（Zn），但通常用ZnO NP包装的食物，如金枪鱼，玉米和芦笋。结果发现，这些食物中的锌含量比推荐的膳食摄入量高出约100倍。使用由Caco-2和HT 29-MTX细胞组成的小肠的体外模型来研究ZnO NP暴露的影响。将细胞暴露于培养基中生理学上现实剂量的原始NP，并暴露于进行体外消化的NP，以更好地反映NP一旦进入人胃肠道就可能经历的转化。铁（Fe），锌，葡萄糖和脂肪酸的摄取和/或运输进行了评估和肠碱性磷酸酶（IAP）水平进行了测量之前和之后NP曝光。研究结果表明，有一个减少75%的铁运输和减少30%的葡萄糖运输后，ZnO NP曝光。这些降低与其转运蛋白的基因表达变化一致。还有证据表明，ZnO NP影响肠细胞的微绒毛，因此减少了可用于吸收营养物质的表面积。这些结果表明，在人体小肠的体外模型中，摄入生理相关剂量的ZnO NP可以改变肠道功能。
Zinc oxide nanoparticles (ZnO NP) may be present in food packaging, which would put consumers at risk of NP ingestion. There is little information on the amount of ZnO NP that are present in food packaging and the effects of ZnO exposure on intestinal function. To estimate physiologically relevant ZnO exposures, foods that are naturally low in zinc (Zn), but are commonly packaged with ZnO NP, such as tuna, corn, and asparagus, were analyzed with inductively coupled plasma mass spectrometry (ICP-MS). It was found that the Zn present in a serving of these foods is approximately one hundred times higher than the recommended dietary allowance. An in vitro model of the small intestine composed of Caco-2 and HT29-MTX cells was used to investigate the effects of ZnO NP exposure. Cells were exposed to physiologically realistic doses of pristine NP in culture medium and to NP subjected to an in vitro digestion to better reflect the transformation that the NP may undergo once they enter the human GI tract. Uptake and/or transport of iron (Fe), Zn, glucose, and fatty acids were assessed and intestinal alkaline phosphatase (IAP) levels were measured before and after NP exposure. The findings show that there is a 75% decrease in Fe transport and a 30% decrease in glucose transport following ZnO NP exposure. These decreases were consistent with gene expression changes for their transporters. There is also evidence that the ZnO NP affect the microvilli of the intestinal cells, therefore reducing the amount of surface area available to absorb nutrients. These results suggest that the ingestion of physiologically relevant doses of ZnO NP can alter intestinal function in an in vitro model of the human small intestine.
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DOI：
10.1504/ijbnn.2013.054515
发表时间：
2013
期刊：
International journal of biomedical nanoscience and nanotechnology
影响因子：
--
作者：
Bergin IL;Witzmann FA
通讯作者：
Witzmann FA
影响因子：
3.9
作者：
Chen, MD;Liou, SJ;Lin, WH
通讯作者：
Lin, WH
影响因子：
5.6
作者：
Alkaladi A;Abdelazim AM;Afifi M
通讯作者：
Afifi M
DOI：
10.1016/b978-0-12-800723-5.00034-6
发表时间：
2016-01-01
期刊：
ANTIMICROBIAL FOOD PACKAGING
影响因子：
--
作者：
Espitia, P. J. P.;Otoni, C. G.;Soares, N. F. F.
通讯作者：
Soares, N. F. F.
影响因子：
13.3
作者：
Chia, Sing Ling;Tay, Chor Yong;Leong, David T.
通讯作者：
Leong, David T.