Induction of reactive oxygen species and the potential role of NADPH oxidase in hyperhydricity of garlic plantlets in vitro
Induction of reactive oxygen species and the potential role of NADPH oxidase in hyperhydricity of garlic plantlets in vitro
复制标题
活性氧的诱导和 NADPH 氧化酶在大蒜试管苗高含水性中的潜在作用
DOI：
10.1007/s00709-016-0957-z
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发表时间：
2016
期刊：
影响因子：
2.9
通讯作者：
Wu Zhen
中科院分区：
文献类型：
--
作者：
Tian Jie;Cheng Yaqi;Kong Xiangyu;Liu Min;Jiang Fangling;Wu Zhen
多水是一种与氧化应激有关的生理性疾病。植物体内活性氧物种(ROS)的产生是由多种酶引发的，包括质膜定位的烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶、细胞壁结合的过氧化物酶(POD)和质外体多胺氧化酶(PAO)。与高水分相关的氧化爆发的来源尚不清楚。为了研究NADPH氧化酶、POD和PAO在ROS产生和高水分中的作用，应用外源过氧化氢(H_2O_2)和各ROS产生酶的抑制剂来探讨大蒜试管苗氧化胁迫诱导的机制。浓度为1.5 mg/L的H_2O_2促进了内源ROS的产生和高水分的发生，并提高了NADPH氧化酶、POD和PAO的活性。在整个处理过程中，NADPH氧化酶活性持续上升，而POD和PAO活性呈现短暂上升后下降的趋势。组织化学和细胞化学显示，每种酶的特异性抑制剂有效地抑制了ROS的积累。此外，过氧化氢胁迫下植株的超氧阴离子生成量、过氧化氢含量和萌发频率均显著下降。NADPH氧化酶抑制剂对超氧阴离子产生的抑制作用最强。结果表明，NADPH氧化酶、POD和PAO参与了内源ROS的诱导。NADPH氧化酶的激活可能在大蒜试管苗在高水分条件下的氧化猝发中起关键作用。
Hyperhydricity is a physiological disorder associated with oxidative stress. Reactive oxygen species (ROS) generation in plants is initiated by various enzymatic sources, including plasma membrane-localized nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, cell wall-bound peroxidase (POD), and apoplastic polyamine oxidase (PAO). The origin of the oxidative burst associated with hyperhydricity remains unknown. To investigate the role of NADPH oxidases, POD, and PAO in ROS production and hyperhydricity, exogenous hydrogen peroxide (H2O2) and inhibitors of each ROS-producing enzyme were applied to explore the mechanism of oxidative stress induction in garlic plantlets in vitro. A concentration of 1.5 mM H2O2increased endogenous ROS production and hyperhydricity occurrence and enhanced the activities of NADPH oxidases, POD, and PAO. During the entire treatment period, NADPH oxidase activity increased continuously, whereas POD and PAO activities exhibited a transient increase and subsequently declined. Histochemical and cytochemical visualization demonstrated that specific inhibitors of each enzyme effectively suppressed ROS accumulation. Moreover, superoxide anion generation, H2O2content, and hyperhydric shoot frequency in H2O2-stressed plantlets decreased significantly. The NADPH oxidase inhibitor was the most effective at suppressing superoxide anion production. The results suggested that NADPH oxidases, POD, and PAO were responsible for endogenous ROS induction. NADPH oxidase activation might play a pivotal role in the oxidative burst in garlic plantlets in vitro during hyperhydricity.
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质外体氧化爆发是大蒜试管苗体内水分过多的关键因素
影响因子：
3
作者：
Tian Jie;Jiang Fangling;Wu Zhen
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
11.6
作者：
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