Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.
Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.
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Gα-cAMP/PKA 信号通路在球毛壳纤维素利用中的调节
DOI：
10.1186/s12934-018-1008-6
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发表时间：
2018-10-11
影响因子：
6.4
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Hu Y;Liu Y;Hao X;Wang D;Akhberdi O;Xiang B;Zhu X
典型的异源三聚体 G 蛋白-cAMP/PKA 途径调节丝状真菌中的许多细胞过程。球毛壳菌 (Chaetomium globosum) 是一种腐生真菌，以产生许多次生代谢产物而闻名，包括细胞毒性毛球蛋白 A (ChA) 以及丰富的纤维素酶和木聚糖酶。在这里，我们报告了 C. globosum 中该信号通路的功能特征。我们通过敲除假定的 Gα 编码基因 gna1（在 pG14 突变体中）来阻断该途径。这导致纤维素酶生产受损并显着降低主要纤维素酶和木聚糖酶基因的转录。几乎所有参与纤维素降解的糖水解酶家族基因均下调，包括主要纤维素酶基因 cel7a、cel6a、egl1 和 egl2。重要的是，转录因子的表达也被发现受到 gna1 的调节，尤其是 Ace1、Clr1/2 和 Hap2/3/5 复合物。此外，RNA-Seq 分析和定量逆转录 (qRT)-PCR 证明，包括淀粉和蔗糖代谢途径在内的碳代谢过程显着减少。有趣的是，这些缺陷可以通过同时敲低编码 cAMP 依赖性 PKA 调节亚基（在 pGP6 突变体中）的 pkaR 基因或补充 cAMP 类似物 8-Br-cAMP 来恢复。此外，调节纤维素酶产生的Gα-cAMP/PKA途径受到包括碳源和光在内的环境信号的调节，其中VelB/VeA/LaeA复合物和ENVOY可能作为下游效应器。这些结果首次揭示了异源三聚体 Gα-cAMP/PKA 途径在调节球藻纤维素酶和木聚糖酶利用中的积极作用。本文的在线版本 (10.1186/s12934-018-1008-6) 包含补充材料，可供授权用户使用。
The canonical heterotrimeric G protein-cAMP/PKA pathway regulates numerous cellular processes in filamentous fungi. Chaetomium globosum, a saprophytic fungus, is known for producing many secondary metabolites, including cytotoxic chaetoglobosin A (ChA), as well as abundant cellulase and xylanase. Here we report on the functional characterization of this signaling pathway in C. globosum. We blocked the pathway by knocking down the putative Gα-encoding gene gna1 (in the pG14 mutant). This led to impaired cellulase production and significantly decreased transcription of the major cellulase and xylanase genes. Almost all the glycohydrolase family genes involved in cellulose degradation were downregulated, including the major cellulase genes, cel7a, cel6a, egl1, and egl2. Importantly, the expression of transcription factors was also found to be regulated by gna1, especially Ace1, Clr1/2 and Hap2/3/5 complex. Additionally, carbon metabolic processes including the starch and sucrose metabolism pathway were substantially diminished, as evidenced by RNA-Seq profiling and quantitative reverse transcription (qRT)-PCR. Interestingly, these defects could be restored by simultaneous knockdown of the pkaR gene encoding the regulatory subunit of cAMP-dependent PKA (in the pGP6 mutant) or supplement of the cAMP analog, 8-Br-cAMP. Moreover, the Gα-cAMP/PKA pathway regulating cellulase production is modulated by environmental signals including carbon sources and light, in which VelB/VeA/LaeA complex and ENVOY probably work as downstream effectors. These results revealed, for the first time, the positive role of the heterotrimeric Gα-cAMP/PKA pathway in the regulation of cellulase and xylanase utilization in C. globosum. The online version of this article (10.1186/s12934-018-1008-6) contains supplementary material, which is available to authorized users.
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作者：
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