Bacteria isolated from bats inhibit the growth of Pseudogymnoascus destructans, the causative agent of white-nose syndrome.
Bacteria isolated from bats inhibit the growth of Pseudogymnoascus destructans, the causative agent of white-nose syndrome.
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DOI：
10.1371/journal.pone.0121329
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kilpatrick AM
中科院分区：
文献类型：
--
作者：
Hoyt JR;Cheng TL;Langwig KE;Hee MM;Frick WF;Kilpatrick AM
新出现的传染病是对野生动物的主要威胁。最近出现了几种皮肤真菌病原体，并导致一些脊椎动物群体广泛死亡，包括两栖动物、蝙蝠、响尾蛇和人类。白鼻综合症是由皮肤真菌病原菌破坏假裸囊菌引起的，它威胁着几种冬眠蝙蝠物种的灭绝，而且有效的治疗策略很少。人们越来越认识到皮肤微生物组在决定疾病结果方面发挥着重要作用。我们从四种蝙蝠的皮肤中分离出细菌，并将这些分离物与破坏性蝙蝠共培养，以鉴定可能抑制或杀死破坏性蝙蝠的细菌。然后，我们用六种细菌分离株（全部属于假单胞菌属）在体外进行了两次相互挑战实验，以量化这些细菌对破坏性假单胞菌生长的影响。与非抑制性对照细菌相比，所有六种假单胞菌分离株均显着抑制破坏性假单胞菌的生长，并且两种分离株在抑制破坏性假单胞菌生长至少 35 天方面明显优于其他分离株。在这两个攻击实验中，破坏性 P. destructans 生长的抑制程度取决于破坏性 P. destructans 的初始浓度和细菌分离株的初始浓度。这些结果表明，在蝙蝠身上天然存在的细菌可以在体外抑制破坏性疟原虫的生长，应该进一步研究其作为可能的益生菌以保护蝙蝠免受白鼻综合症的影响。此外，这些细菌的存在可能会影响个体、群体和物种的疾病结果。
Emerging infectious diseases are a key threat to wildlife. Several fungal skin pathogens have recently emerged and caused widespread mortality in several vertebrate groups, including amphibians, bats, rattlesnakes and humans. White-nose syndrome, caused by the fungal skin pathogen Pseudogymnoascus destructans, threatens several hibernating bat species with extinction and there are few effective treatment strategies. The skin microbiome is increasingly understood to play a large role in determining disease outcome. We isolated bacteria from the skin of four bat species, and co-cultured these isolates with P. destructans to identify bacteria that might inhibit or kill P. destructans. We then conducted two reciprocal challenge experiments in vitro with six bacterial isolates (all in the genus Pseudomonas) to quantify the effect of these bacteria on the growth of P. destructans. All six Pseudomonas isolates significantly inhibited growth of P. destructans compared to non-inhibitory control bacteria, and two isolates performed significantly better than others in suppressing P. destructans growth for at least 35 days. In both challenge experiments, the extent of suppression of P. destructans growth was dependent on the initial concentration of P. destructans and the initial concentration of the bacterial isolate. These results show that bacteria found naturally occurring on bats can inhibit the growth of P. destructans in vitro and should be studied further as a possible probiotic to protect bats from white-nose syndrome. In addition, the presence of these bacteria may influence disease outcomes among individuals, populations, and species.
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影响因子：
14.9
作者：
EDWARDS, U;ROGALL, T;BOTTGER, EC
通讯作者：
BOTTGER, EC
影响因子：
8.8
作者：
Langwig, Kate E.;Frick, Winifred F.;Kilpatrick, A. Marm
通讯作者：
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影响因子：
11
作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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