A systemic study of indoxacarb resistance in Spodoptera litura revealed complex expression profiles and regulatory mechanism
A systemic study of indoxacarb resistance in Spodoptera litura revealed complex expression profiles and regulatory mechanism
复制标题
斜纹夜蛾茚虫威抗性的系统研究揭示了复杂的表达谱和调控机制
DOI：
10.1038/s41598-019-51234-5
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发表时间：
2019-10
影响因子：
4.6
通讯作者：
Liao Xiaolan
中科院分区：
文献类型：
--
作者：
Shi Li;Shi Yao;Zhang Ya;Liao Xiaolan
斜纹夜蛾（Spodoptera litura）是世界范围内重要的农作物和蔬菜害虫，其抗药性发展迅速。然而，该害虫的抗性机制仍不清楚。在这项研究中，基因和miRNA的变化在敏感，抗药性和田间抗药性菌株的S。litura的特征。共检测到910个共上调基因和737个共下调基因。进一步分析表明，细胞色素P450、羧基/胆碱酯酶、谷胱甘肽S-转移酶、ATP结合盒转运蛋白、UDP-葡萄糖醛酸转移酶、氨肽酶N、唾液酸、丝氨酸蛋白酶和角质层蛋白等126个共差异表达基因（co-DEGs）可能在抗茚虫威的过程中起重要作用。斜纹。此外，共鉴定出91个已知的miRNAs和52个新的miRNAs，其中10个miRNAs在耐药菌株中存在共差异表达。斜纹。此外，10个共同差异表达的miRNAs（co-DEmiRNAs）根据预期的miRNA-mRNA负调控模式预测了co-DEGs，预测了37个与茚虫威抗性相关的co-DEGs为靶基因。这些结果不仅通过揭示复杂的特征拓宽了我们对杀虫剂抗性分子机制的认识，而且为进一步研究参与S. indoxacarb抗性的miRNA机制提供了重要线索。斜纹。
The tobacco cutworm, Spodoptera litura, is an important pest of crop and vegetable plants worldwide, and its resistance to insecticides have quickly developed. However, the resistance mechanisms of this pest are still unclear. In this study, the change in mRNA and miRNA profiles in the susceptible, indoxacarb-resistant and field indoxacarb-resistant strains of S. litura were characterized. Nine hundred and ten co-up-regulated and 737 co-down-regulated genes were identified in the resistant strains. Further analysis showed that 126 co-differentially expressed genes (co-DEGs) (cytochrome P450, carboxy/cholinesterase, glutathione S-transferase, ATP-binding cassette transporter, UDP-glucuronosyl transferase, aminopeptidase N, sialin, serine protease and cuticle protein) may play important roles in indoxacarb resistance in S. litura. In addition, a total of 91 known and 52 novel miRNAs were identified, and 10 miRNAs were co-differentially expressed in the resistant strains of S. litura. Furthermore, 10 co-differentially expressed miRNAs (co-DEmiRNAs) had predicted co-DEGs according to the expected miRNA-mRNA negative regulation pattern and 37 indoxacarb resistance-related co-DEGs were predicted to be the target genes. These results not only broadened our understanding of molecular mechanisms of insecticide resistance by revealing complicated profiles, but also provide important clues for further study on the mechanisms of miRNAs involved in indoxacarb resistance in S. litura.
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