Co-culture with Listeria monocytogenes within a dual-species biofilm community strongly increases resistance of Pseudomonas putida to benzalkonium chloride.
Co-culture with Listeria monocytogenes within a dual-species biofilm community strongly increases resistance of Pseudomonas putida to benzalkonium chloride.
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与双物种生物膜群落中的李斯特菌单核细胞增生植物共同培养可强烈提高假单胞菌对苯扎核氯化物的耐药性。
DOI：
10.1371/journal.pone.0077276
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nychas GJ
中科院分区：
文献类型：
--
作者：
Giaouris E;Chorianopoulos N;Doulgeraki A;Nychas GJ
生物膜形成是一种几乎在微生物和表面存在紧密接近的地方发生的现象。本研究旨在评估细菌相互作用对单核细胞增生李斯特菌和恶臭假单胞菌在不锈钢（SS）上形成双物种生物膜群落的能力的可能影响，以及对随后的固着细胞对苯扎氯铵（BC）的抗性的影响。混合培养生物膜的可能的渐进适应性BC也进行了研究。为了实现这些，每个物种留下3个菌株以在SS试样上形成混合培养物生物膜，在单物种或双物种条件下在每日可再生生长培养基中孵育总共10天。每天，将生物膜细胞暴露于消毒处理。结果表明，L。单核细胞增多症强烈增加恶臭假单胞菌生物膜细胞对BC的抗性，而培养条件（单/双种）似乎没有显著影响L.单核细胞增生性生物膜细胞。BC主要杀死L.当在整个孵育期间将其应用于双物种固着群落时，单核细胞增多症细胞的数量增加，尽管事实上从第2天起该群落主要由恶臭假单胞菌细胞组成（>90%）。两种L均未观察到对BC的明显适应。单核细胞增多症或恶臭假单胞菌生物膜细胞。脉冲场凝胶电泳（PFGE）分析表明，不同的菌株表现不同的生物膜形成和抗菌药物的耐药性。混合培养生物膜的生理行为的这种知识可以提供必要的信息来控制它们的形成。
Biofilm formation is a phenomenon occurring almost wherever microorganisms and surfaces exist in close proximity. This study aimed to evaluate the possible influence of bacterial interactions on the ability of Listeria monocytogenes and Pseudomonas putida to develop a dual-species biofilm community on stainless steel (SS), as well as on the subsequent resistance of their sessile cells to benzalkonium chloride (BC) used in inadequate (sub-lethal) concentration (50 ppm). The possible progressive adaptability of mixed-culture biofilms to BC was also investigated. To accomplish these, 3 strains per species were left to develop mixed-culture biofilms on SS coupons, incubated in daily renewable growth medium for a total period of 10 days, under either mono- or dual-species conditions. Each day, biofilm cells were exposed to disinfection treatment. Results revealed that the simultaneous presence of L. monocytogenes strongly increased the resistance of P. putida biofilm cells to BC, while culture conditions (mono-/dual-species) did not seem to significantly influence the resistance of L. monocytogenes biofilm cells. BC mainly killed L. monocytogenes cells when this was applied against the dual-species sessile community during the whole incubation period, despite the fact that from the 2nd day this community was mainly composed (>90%) of P. putida cells. No obvious adaptation to BC was observed in either L. monocytogenes or P. putida biofilm cells. Pulsed field gel electrophoresis (PFGE) analysis showed that the different strains behaved differently with regard to biofilm formation and antimicrobial resistance. Such knowledge on the physiological behavior of mixed-culture biofilms could provide the information necessary to control their formation.
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影响因子：
4.4
作者：
Behnke, Sabrina;Parker, Albert E.;Camper, Anne K.
通讯作者：
Camper, Anne K.
影响因子：
2.8
作者：
Dynes, James J.;Lawrence, John R.;Hitchcock, Adam P.
通讯作者：
Hitchcock, Adam P.
影响因子：
5.3
作者：
Cruz, Cristina D.;Fletcher, Graham C.
通讯作者：
Fletcher, Graham C.
影响因子：
5.4
作者：
Bagge-Ravn, D;Ng, Y;Gram, L
通讯作者：
Gram, L
DOI：
10.1016/j.ijfoodmicro.2004.03.031
发表时间：
2004-12-15
影响因子：
5.4
作者：
Carpentier, B;Chassaing, D
通讯作者：
Chassaing, D