Direct cell-cell contact between mesenchymal stem cells and endothelial progenitor cells induces a pericyte-like phenotype in vitro.
Direct cell-cell contact between mesenchymal stem cells and endothelial progenitor cells induces a pericyte-like phenotype in vitro.
复制标题
DOI：
10.1155/2014/395781
复制
发表时间：
2014
影响因子：
--
通讯作者：
Verrier S
中科院分区：
文献类型：
--
作者：
Loibl M;Binder A;Herrmann M;Duttenhoefer F;Richards RG;Nerlich M;Alini M;Verrier S
组织工程技术用于再生大型骨缺损需要对所应用的构建物进行充分的血管化以确保足够的氧气和营养供应。在我们之前的工作中，通过骨髓来源的干细胞(MSCs)和内皮祖细胞(EPC)的共培养成功地建立了预血管化的3D支架。我们发现稳定周细胞(PC)是新形成的毛细血管样结构的一部分。在目前的研究中，我们报告了MSCs和EPC之间相互作用导致周细胞样细胞分化的初步数据。骨髓间充质干细胞和内皮祖细胞接种于Transwell培养、直接共培养和单独培养。细胞在IMDM 10%FCS或IMDM 5%FCS 5%血小板裂解液中培养10天。分别于培养第0、3、7、10天用逆转录聚合酶链式反应分析PC标志物CD146、NG2、αSMA和PDGFR-β的基因表达。这些结果提示周细胞样细胞来源于间充质干细胞，细胞与内皮祖细胞接触是分化过程中的一个重要因素。这些发现强调了共培养策略的概念，以促进基于细胞的组织工程骨移植的血管生成。
Tissue engineering techniques for the regeneration of large bone defects require sufficient vascularisation of the applied constructs to ensure a sufficient supply of oxygen and nutrients. In our previous work, prevascularised 3D scaffolds have been successfully established by coculture of bone marrow derived stem cells (MSCs) and endothelial progenitor cells (EPCs). We identified stabilising pericytes (PCs) as part of newly formed capillary-like structures. In the present study, we report preliminary data on the interactions between MSCs and EPCs, leading to the differentiation of pericyte-like cells. MSCs and EPCs were seeded in transwell cultures, direct cocultures, and single cultures. Cells were cultured for 10 days in IMDM 10% FCS or IMDM 5% FCS 5% platelet lysate medium. Gene expression of PC markers, CD146, NG2, αSMA, and PDGFR-β, was analysed using RT-PCR at days 0, 3, 7, and 10. The upregulation of CD146, NG2, and αSMA in MSCs in direct coculture with EPCs advocates the MSCs' differentiation towards a pericyte-like phenotype in vitro. These results suggest that pericyte-like cells derive from MSCs and that cell-cell contact with EPCs is an important factor for this differentiation process. These findings emphasise the concept of coculture strategies to promote angiogenesis for cell-based tissue engineered bone grafts.
登录
查看更多内容
影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
3.3
作者：
Lippross, Sebastian;Loibl, Markus;Verrier, Sophie
通讯作者：
Verrier, Sophie
影响因子：
4.8
作者：
Bondjers, Cecilia;He, Liqun;Betsholtz, Christer
通讯作者：
Betsholtz, Christer
影响因子：
2.6
作者：
Covas, Dimas T.;Panepucci, Rodrigo A.;Zago, Marco A.
通讯作者：
Zago, Marco A.
影响因子：
7.3
作者：
Li, Q;Yu, Y;Olsen, BR
通讯作者：
Olsen, BR