Early growth response protein 1 promotes restenosis by upregulating intercellular adhesion molecule-1 in vein graft.
Early growth response protein 1 promotes restenosis by upregulating intercellular adhesion molecule-1 in vein graft.
复制标题
早期生长反应蛋白 1 通过上调静脉移植物中的细胞间粘附分子 1 促进再狭窄。
DOI：
10.1155/2013/432409
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发表时间：
2013
影响因子：
--
通讯作者：
Du J
中科院分区：
文献类型：
--
作者：
Zhang K;Cao J;Dong R;Du J
目标.目的探讨Egr-1与移植静脉再狭窄的关系及相关机制。方法.在Egr-1敲除（KO）和野生型（WT）小鼠中建立小鼠静脉移植物模型。取移植静脉进行病理学检查和免疫组化分析。内皮细胞（EC）的刺激，通过使用一个计算机控制的循环应力单元。BrdU染色检测ECs增殖活性，PCR检测Egr-1和ICAM-1 mRNA表达。Western-blot检测Egr-1和细胞间粘附分子-1（ICAM-1）蛋白的表达。结果Egr-1基因敲除小鼠移植静脉的管腔比野生型小鼠宽。Egr-1基因敲除可抑制机械牵张刺激后的ECs增殖（P < 0.05）。机械牵张刺激后，移植静脉和内皮细胞Egr-1和ICAM-1的mRNA和蛋白表达均增加（P < 0.05）。Egr-1基因敲除小鼠内皮细胞ICAM-1表达明显降低（P < 0.05）。结论. Egr-1可能通过上调ICAM-1的表达而促进内皮细胞增殖，导致移植静脉再狭窄。作为静脉移植物再狭窄的关键因素，它可能成为预防CABG术后再狭窄的靶点。
Objectives. To verify the relationship between Egr-1 and vein graft restenosis and investigate the related mechanisms. Methods. Mouse vein graft models were established in Egr-1 knockout (KO) and wild-type (WT) mice. The vein grafts in the mice were taken for pathological examination and immunohistochemical analysis. The endothelial cells (ECs) were stimulated by using a computer-controlled cyclic stress unit. BrdU staining and PCR were used to detect ECs proliferation activity and Egr-1 and ICAM-1 mRNA expression, respectively. Western-blot analysis was also used to detect expression of Egr-1 and intercellular adhesion molecule-1 (ICAM-1) proteins. Results. The lumens of vein grafts in Egr-1 KO mice were wider than in WT mice. ECs proliferation after mechanical stretch stimulation was suppressed by Egr-1 knockout (P < 0.05). Both in vein grafts and ECs from WT mice after mechanical stretch stimulation, mRNA expression and protein of Egr-1 and ICAM-1 showed increases (P < 0.05). However, ICAM-1 expression was significantly suppressed in ECs from Egr-1 knockout mice (P < 0.05). Conclusions. Egr-1 may promote ECs proliferation and result in vein graft restenosis by upregulating the expression of ICAM-1. As a key factor of vein graft restenosis, it could be a target for the prevention of restenosis after CABG surgery.
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影响因子：
2.2
作者：
Li, Fan Dong;Sexton, Kevin W.;Hocking, Kyle M.;Osgood, Michael J.;Eagle, Susan;Cheung-Flynn, Joyce;Brophy, Colleen M.;Komalavilas, Padmini
通讯作者：
Komalavilas, Padmini
影响因子：
4.6
作者：
Hinokiyama, Kazuhiro;Valen, Guro;Vaage, Jarle
通讯作者：
Vaage, Jarle
影响因子：
3.4
作者：
Rahimi, K;Maerz, HK;Tárnok, A
通讯作者：
Tárnok, A
DOI：
10.3109/14767058.2012.736564
发表时间：
2013-03
期刊：
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
影响因子：
--
作者：
Mordwinkin NM;Ouzounian JG;Yedigarova L;Montoro MN;Louie SG;Rodgers KE
通讯作者：
Rodgers KE
影响因子：
5.3
作者：
GIUS, D;CAO, XM;SUKHATME, VP
通讯作者：
SUKHATME, VP