The basis of pod dehiscence: anatomical traits of the dehiscence zone and expression of eight pod shatter-related genes in four species of Brassicaceae
The basis of pod dehiscence: anatomical traits of the dehiscence zone and expression of eight pod shatter-related genes in four species of Brassicaceae
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结荚开裂的基础：十字花科四种植物开裂区的解剖特征及8个破荚相关基因的表达
DOI：
10.1007/s10535-016-0599-1
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发表时间：
2016-03
影响因子：
1.5
通讯作者：
Wang JB
中科院分区：
文献类型：
--
作者：
Zhang Y;Shen YY;Wu XM;Wang JB
十字花科的成员通过通常称为果实开裂或豆荚破碎的机制传播种子。荚果破碎受到瓣膜边缘结构变化以及与瓣膜发育相关的分子控制途径的影响。使用组织学染色比较了甘蓝型油菜、甘蓝型油菜、甘蓝型和白芥子的开裂区的解剖模式，这些代表了抗破荚能力不同的果实类型。易碎荚的植物甘蓝型油菜在其维管束处显示出木质素沉积增加以及细胞层分离增加。在抗荚果破碎植物 S. alba、B. rapa 和 B. carinata 中，我们观察到两层木质化的瓣膜边缘细胞。从这四个物种中，我们分离并鉴定了参与果实发育和荚果破碎的 SHATTERPROOF (SHP1、SHP2)、INDEHISCENT (IND)、ALCATRAZ (ALC)、FRUITFULL (FUL)、AGAMOUS (AG)、NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) 和 SEEDSTICK (STK) 基因的同源物拟南芥。通过实时聚合酶链式反应对这八个基因进行转录分析，结果表明这八个基因表达模式的差异可能与这四个物种内的开裂变异有关。
Members of the Brassicaceae family disperse their seeds through a mechanism commonly referred to as fruit dehiscence or pod shatter. Pod shatter is influenced by variations in valve margin structure and by the molecular control pathways related to valve development. Anatomical patterns of the dehiscence zone from Brassica napus L., Brassica rapa L., Brassica carinata L., and Sinapis alba L., representing fruit types differing in pod shatter resistance, were compared using histological staining. The pod shatter-susceptible plant B. napus showed an increased lignin deposition at the vascular bundle of the replum as well as an increased separation of cell layers. In pod shatter-resistant plants S. alba, B. rapa, and B. carinata, we observed two layers of lignified valve margin cells. From these four species, we isolated and identified homologs of SHATTERPROOF (SHP1, SHP2), INDEHISCENT (IND), ALCATRAZ (ALC), FRUITFULL (FUL), AGAMOUS (AG), NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1), and SEEDSTICK (STK) genes involved in fruit development and pod shatter in Arabidopsis. Transcriptional analysis of these eight genes was performed by real-time polymerase chain reaction and the results demonstrate that differences in the expression patterns of the eight genes may be associated with dehiscence variation within these four species.
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影响因子：
2
作者：
Wang, R.;Ripley, V. L.;Rakow, G.
通讯作者：
Rakow, G.
影响因子：
6.9
作者：
Seymour GB;Granell A
通讯作者：
Granell A
影响因子：
4.5
作者：
González-Reig S;Ripoll JJ;Vera A;Yanofsky MF;Martínez-Laborda A
通讯作者：
Martínez-Laborda A
DOI：
10.1046/j.1365-2818.1996.111391.x
发表时间：
1996-02-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
Spence, J;Vercher, Y;Harris, N
通讯作者：
Harris, N
影响因子：
64.8
作者：
Pinyopich, A;Ditta, GS;Yanofsky, MF
通讯作者：
Yanofsky, MF