Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice.
Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice.
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铁皮石斛独特多糖标记物对BALB/c小鼠的抗疲劳作用
DOI：
10.3390/molecules22010155
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发表时间：
2017-01-18
期刊：
影响因子：
--
通讯作者：
Han QB
中科院分区：
文献类型：
--
作者：
Wei W;Li ZP;Zhu T;Fung HY;Wong TL;Wen X;Ma DL;Leung CH;Han QB
铁皮石斛提取物具有较强的抗疲劳作用；然而，造成这些影响的活性物质仍未确定。一种分子量为730 kDa的葡甘露聚糖（DOP）被鉴定为这种昂贵草本植物的唯一鉴定标记。在我们之前的研究中，DOP对巨噬细胞和淋巴细胞有免疫调节作用。临床报告还显示，患有疲劳综合症的人免疫系统紊乱。由于DOP是铁皮草中独特的主要成分，我们假设DOP也可能具有抗疲劳活性。本研究以红景天提取物为阳性对照，评价DOP对BALB/c小鼠的抗疲劳活性。分别以50 mg/kg和100 mg/kg剂量口服DOP和红景天提取物4周，采用负重游泳试验评价DOP对BALB/c小鼠的抗疲劳活性。测定血清中乳酸脱氢酶（LDH）、肌酸磷酸激酶（CK）、甘油三酯（TG）、血尿素氮（BUN）、超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸（LD）、谷胱甘肽过氧化物酶（GSH-Px）、肝脏糖原和腓肠肌糖原的含量。采用四氮唑化合物（MTS）法测定其对T细胞和B细胞变异性的影响。负重游泳运动引起疲劳综合征，主要表现为血清SOD/GSH-Px、腓肠肌糖原降低，血清LDH、BUN、MDA、CK、TG、LD升高。dopp和红景天提取物均能在一定程度上抑制小鼠疲劳指标；但其作用明显强于红景天提取物。与阳性对照相比，服用DOP的小鼠耐力、体重和食物摄入量均有所增加。此外，与对照组相比，dopa喂养小鼠的T淋巴细胞和B淋巴细胞的细胞变异性显著增加。本研究表明，officinale独特的优势多糖DOP比红景天提取物具有更强的抗疲劳活性。因此，DOP在药物开发中具有很大的潜力，可以成为减少疲劳的保健产品。
Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D. officinale, we hypothesize that DOP may also have anti-fatigue activity. The present study aims to evaluate the anti-fatigue activity of DOP on BALB/c mice, with Rhodiola rosea extract as a positive control. DOP and Rhodiola rosea extract were orally administered at doses of 50 mg/kg and 100 mg/kg, respectively, for four weeks, and the anti-fatigue activity of DOP on BALB/c mice was evaluated using the weight-loaded swimming test. The contents of lactic dehydrogenase (LDH), creatine phosphokinase (CK), triglyceride (TG), blood urea nitrogen (BUN), superoxide dismutase (SOD), malondialdehyde (MDA), lactic acid (LD), and glutathione peroxidase (GSH-Px) in serum, glycogen of liver and gastrocnemius muscle were also determined. Their effects on variability of T cells and B cells were determined by using tetrazolium compound (MTS) method. The weight-loaded swimming exercise caused fatigue syndrome, mainly including the decreases of serum SOD/GSH-Px and gastrocnemius glycogen, as well as the increases of LDH, BUN, MDA, CK, TG, and LD in serum. All of these indicators of fatigue were inhibited to a certain extent by both DOP and Rhodiola rosea extract; however, the effects of DOP were much stronger than those of Rhodiola rosea extract. Compared to the positive control, mice dosed with DOP showed increases in endurance, body weight, and food intake. Furthermore, DOP-feeding mice significantly increased the cell variability of T lymphocytes and B lymphocytes, compared with that of mice in control group. This study indicates that the unique and dominant polysaccharide DOP of D. officinale has stronger anti-fatigue activity than Rhodiola rosea extract. As such, DOP has promising potential for pharmaceutical development into health products to reduce fatigue.
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