Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution.
Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution.
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DOI：
10.3390/s23187762
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发表时间：
2023-09-08
期刊：
影响因子：
--
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Hu Q;Jia H;Wang Y;Xu S
核酸是分子传感的主要目标，因为它们广泛参与生物功能。因此，确定它们的存在、运动和结合特异性是很好的追求。然而，目前的许多技术通常是复杂的，昂贵的，往往缺乏单核苷酸分辨率。在本文中，我们报告的力诱导可视化方法，依赖于新的概念，机械力，以确定功能的核酸位置与单核苷酸分辨率。使用可调节的机械力克服了生物环境中常见的分析物浓度的变化和缓冲液条件的差异。两个例子来验证该方法：一个是探测核糖体易位过程中的mRNA运动，另一个是揭示DNA结合药物的相互作用位点和强度的基础上的力的振幅。该方法的灵活性、相关装置的简单性和多重检测的能力将潜在地使广泛的生物医学应用成为可能。
Nucleic acids are major targets for molecular sensing because of their wide involvement in biological functions. Determining their presence, movement, and binding specificity is thus well pursued. However, many current techniques are usually sophisticated, expensive, and often lack single-nucleotide resolution. In this paper, we report the force-induced visualization method that relies on the novel concept of mechanical force to determine the functional positions of nucleic acids with single-nucleotide resolution. The use of an adjustable mechanical force overcomes the variation of analyte concentration and differences in buffer conditions that are common in biological settings. Two examples are described to validate the method: one is probing the mRNA movement during ribosomal translocation, and the other is revealing the interacting sites and strengths of DNA-binding drugs based on the force amplitude. The flexibility of the method, simplicity of the associated device, and capability of multiplexed detection will potentially enable a broad range of biomedical applications.
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影响因子：
64.8
作者：
Bustamante, C;Bryant, Z;Smith, SB
通讯作者：
Smith, SB
影响因子：
14.9
作者：
MCCLELLAND, M;NELSON, M;RASCHKE, E
通讯作者：
RASCHKE, E
影响因子：
3.4
作者：
Halvorsen, Ken;Wong, Wesley P.
通讯作者：
Wong, Wesley P.
DOI：
10.1039/c5cc10535h
发表时间：
2016-03-04
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Hu Q;Yang H;Wang Y;Xu S
通讯作者：
Xu S
影响因子：
16.6
作者：
Hu, Qiongzheng;Xu, Shoujun
通讯作者：
Xu, Shoujun