Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration.
Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration.
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DOI：
10.1007/s00216-013-7159-8
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发表时间：
2013-09
影响因子：
4.3
通讯作者：
Huestis, Marilyn A.
中科院分区：
文献类型：
--
作者：
Lee, Dayong;Vandrey, Ryan;Milman, Garry;Bergamaschi, Mateus;Mendu, Damodara R.;Murray, Jeannie A.;Barnes, Allan J.;Huestis, Marilyn A.
口服液（OF）是临床和法医药物检测的一种有价值的生物替代品。评估血浆大麻素比率（OF/P）为药物处置和影响基质之间分配的因素提供了重要的药代动力学数据。11名长期吸食大麻的人在一个封闭的研究单位住了51天。有4个为期5天的疗程，分别为0、30、60和120毫克口服Δ9-tetrahydrocannabinol (THC)/天，然后在第5天进行5次抽大麻挑战。每次会议之间有9天的自由吸食大麻。分析OF和血浆标本中四氢大麻酚及其代谢物。在随意吸烟期间，在最后一次吸烟后1小时内，OF/P THC比率很高（中位数为6.1，范围为0.2 - 348.5），在13.0-17.1小时内下降到0.1-20.7（中位数为2.1）。在口服THC的5天内，OF/P THC比率也有所下降，在吸烟后0.25小时，OF/P THC比率中位数从1.4-5.5（0.04-245.6）下降到0.12-0.17（0.04-5.1）。在其他研究中，长时间暴露于更强效的大麻烟雾和口腔大麻喷雾剂与增加的OF/P THC峰值比率有关。中位OF/ p11 -no -9-羧基thc （THCCOOH）比值为0.3-2.5（范围0.1-14.7）ng/µg，随着时间的推移，在不同的给药条件下更加一致。吸烟后口腔污染或口腔喷淋大麻素产品对OF/P THC的比值有显著影响，但对thcooh没有影响，随后呈时间依赖性下降。建立OF和血浆大麻素浓度之间的关系对于从OF浓度推断损伤或其他临床结果至关重要。
Oral fluid (OF) is a valuable biological alternative for clinical and forensic drug testing. Evaluating OF to plasma (OF/P) cannabinoid ratios provides important pharmacokinetic data on the disposition of drug and factors influencing partition between matrices. Eleven chronic cannabis smokers resided on a closed research unit for 51 days. There were four 5-day sessions of 0, 30, 60, and 120 mg oral Δ9-tetrahydrocannabinol (THC)/per day followed by a 5-puff smoked cannabis challenge on Day 5. Each session was separated by 9 days ad-libitum cannabis smoking. OF and plasma specimens were analyzed for THC and metabolites. During ad-libitum smoking, OF/P THC ratios were high (median 6.1, range 0.2– 348.5) within 1 h after last smoking, decreasing to 0.1–20.7 (median 2.1) by 13.0–17.1 h. OF/P THC ratios also decreased during 5-days oral THC dosing, and after the smoked cannabis challenge, median OF/P THC ratios decreased from 1.4–5.5 (0.04–245.6) at 0.25 h to 0.12–0.17 (0.04–5.1) at 10.5 h post smoking. In other studies, longer exposure to more potent cannabis smoke and oromucosal cannabis spray was associated with increased OF/P THC peak ratios. Median OF/P 11-nor-9-carboxy-THC (THCCOOH) ratios were 0.3–2.5 (range 0.1–14.7) ng/µg, much more consistent in various dosing conditions over time. OF/P THC, but not THCCOOH, ratios were significantly influenced by oral cavity contamination after smoking or oromucosal spray of cannabinoid products, followed by time-dependent decreases. Establishing relationships between OF and plasma cannabinoid concentrations is essential for making inferences of impairment or other clinical outcomes from OF concentrations.
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影响因子：
9.3
作者：
Bosker WM;Huestis MA
通讯作者：
Huestis MA
影响因子：
2.5
作者：
Kauert, Gerold F.;Ramaekers, Johannes G.;Toennes, Stefan W.
通讯作者：
Toennes, Stefan W.
影响因子：
9.3
作者：
Lee, Dayong;Schwope, David M.;Huestis, Marilyn A.
通讯作者：
Huestis, Marilyn A.
影响因子：
2.1
作者：
Samyn, N;van Haeren, C
通讯作者：
van Haeren, C
影响因子：
4.2
作者：
Lee, Dayong;Karschner, Erin L.;Milman, Garry;Barnes, Allan J.;Goodwin, Robert S.;Huestis, Marilyn A.
通讯作者：
Huestis, Marilyn A.