Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways.
Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways.
复制标题
DOI：
10.1038/s41598-018-32861-w
复制
发表时间：
2018-09-27
影响因子：
4.6
通讯作者：
Ishida T
中科院分区：
文献类型：
--
作者：
Emam SE;Ando H;Lila ASA;Shimizu T;Okuhira K;Ishima Y;Mahdy MA;Ghazy FS;Sagawa I;Ishida T
我们最近表明，细胞与不同组成的脂质体的体外孵育可以增加外泌体分泌并增加收获的外泌体（细胞外囊泡，EV）的产量。这可能会促进其潜在的治疗实施。在目前的研究中，我们研究了收获的外泌体（EV）的表面蛋白和摄取，以观察细胞与脂质体的孵育是否会改变这些外泌体（EV）的生物学特性。有趣的是，由固体阳离子脂质体诱导的外泌体（EV）缺乏一些主要的外泌体标志物蛋白，如CD9、flotillin-1、膜联蛋白-A2和EGF，并且随后在与供体癌细胞再孵育时具有较低水平的细胞摄取。然而，在正常条件下和通过流体阳离子脂质体诱导的外泌体（EV）显示了整个蛋白质谱，并且表现出供体癌细胞的更高摄取。虽然内吞作用是肿瘤细胞摄取外泌体的主要途径，但内吞作用可以通过多种机制发生。在供体B16BL6细胞中观察到比同种异体C26细胞更高的外泌体摄取，表明供体细胞可能与它们的外泌体（EV）特异性相互作用并将它们贪婪地内化。总之，这些结果表明了一种通过将供体癌细胞与具有不同理化性质的脂质体一起孵育来控制分泌的外泌体（EV）的特征的技术。
We recently showed that in vitro incubation of cells with liposomes of varying compositions can increase exosome secretion and increase the yield of harvested exosomes (extracellular vesicles, EVs). This might foster their potential therapeutic implementations. In the current study, we investigated the surface proteins and the uptake of the harvested exosomes (EVs) to see if the incubation of cells with liposomes would change the biological properties of these exosomes (EVs). Interestingly, exosomes (EVs) induced by solid cationic liposomes lacked some major exosome marker proteins such as CD9, flotillin-1, annexin-A2 and EGF, and subsequently had lower levels of cellular uptake upon re-incubation with donor cancer cells. However, exosomes (EVs) induced under normal condition and by fluid cationic liposomes, displayed the entire spectrum of proteins, and exhibited higher uptake by the donor cancer cells. Although endocytosis was the major uptake pathway of exosomes (EVs) by tumor cells, endocytosis could occur via more than one mechanism. Higher exosome uptake was observed in donor B16BL6 cells than in allogeneic C26 cells, indicating that donor cells might interact specifically with their exosomes (EVs) and avidly internalize them. Taken together, these results suggest a technique for controlling the characteristics of secreted exosomes (EVs) by incubating donor cancer cells with liposomes of varying physiochemical properties.
登录
查看更多内容
影响因子：
46.9
作者：
Alvarez-Erviti, Lydia;Seow, Yiqi;Wood, Matthew J. A.
通讯作者：
Wood, Matthew J. A.
DOI：
10.1016/j.jconrel.2015.07.030
发表时间：
2015-12-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Batrakova EV;Kim MS
通讯作者：
Kim MS
外泌体作为帕金森氏病疗法的药物。
DOI：
10.1016/j.jconrel.2015.03.033
发表时间：
2015-06-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Haney MJ;Klyachko NL;Zhao Y;Gupta R;Plotnikova EG;He Z;Patel T;Piroyan A;Sokolsky M;Kabanov AV;Batrakova EV
通讯作者：
Batrakova EV
影响因子：
7.3
作者：
French KC;Antonyak MA;Cerione RA
通讯作者：
Cerione RA
影响因子：
4
作者：
Otto, Grant P.;Nichols, Benjamin J.
通讯作者：
Nichols, Benjamin J.