Fluorescent probes for the imaging of lipid droplets in live cells
Fluorescent probes for the imaging of lipid droplets in live cells
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用于活细胞中脂滴成像的荧光探针
DOI：
10.1016/j.ccr.2020.213577
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发表时间：
2021-01
影响因子：
20.6
通讯作者：
Xiao-Peng He
中科院分区：
文献类型：
--
作者：
He Tian;Adam C. Sedgwick;Hai-Hao Han;Sajal Sen;Guo-Rong Chen;Yi Zang;Jonathan L. Sessler;Tony D. James;Jia Li;Xiao-Peng He
脂滴（LD）是维持脂质和能量稳态所必需的细胞器。曾经被认为仅仅是惰性的脂肪颗粒，现在被认为是高度动态的，移动的细胞器，需要防止脂毒性和相互作用，并与各种细胞器合作。尽管在理解最不发达国家的作用方面取得了进展，但一些基本问题仍然没有答案。有效的成像剂观察细胞中LD的形态和动态生理过程，可以帮助解决这一知识空白。这些探针有望帮助我们了解LD，并促进新的有效治疗方法的开发。在这篇综述中，我们提供了一个简单的介绍，形成和生理功能的LD，试图突出这些未被重视的细胞器的重要性。最近的例子LD为基础的荧光探针进行了讨论，包括在其设计中使用的荧光现象。迄今为止，分子内电荷转移（ICT）和聚集诱导发射（AIE）荧光机制已被用来创建LD探针。然而，可以设想替代战略。我们希望读者能够了解这些关键细胞器的重要性，准备利用现有的探针探索各种生物学应用，并受到启发，创造新的LD荧光传感器，以进一步了解LD及其相关生理学。
Lipid droplets (LDs) are cellular organelles that are essential for maintaining lipid and energy homeostasis. Once regarded as merely inert fat particles, they are now recognized as highly dynamic, mobile organelles required for preventing lipotoxicity and for interacting and cooperating with various organelles. Despite the progress made in understanding the role of LDs, a number of fundamental questions remain unanswered. Effective imaging agents for observing the morphology and dynamic physiological processes of LDs in cells could help address this knowledge gap. Such probes are expected to aid in our understanding of LDs and facilitate the development of new and effective therapeutics. In this review, we have provided a brief introduction to the formation and physiological functions of LDs in an attempt to highlight the importance of these underappreciated organelles. Recent examples of LD-based fluorescent probes are discussed, including the fluorescence phenomenon used in their design. To date, both intramolecular charge transfer (ICT) and aggregation-induced emission (AIE) fluorescence mechanisms have been exploited to create LD probes. However, alternative strategies can be envisioned. We hope the readers will be enlightened as to the importance of these key organelles, will be poised to exploit existing probes to explore various biological applications, and be inspired to create new LD fluorescent sensors that will further our understanding of LDs and their associated physiology.
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影响因子：
9
作者：
通讯作者：
--
DOI：
10.1083/jcb.200811005
发表时间：
2009-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Rocha N;Kuijl C;van der Kant R;Janssen L;Houben D;Janssen H;Zwart W;Neefjes J
通讯作者：
Neefjes J
影响因子：
5.6
作者：
Koizume S;Miyagi Y
通讯作者：
Miyagi Y
影响因子：
4.4
作者：
Scha.
通讯作者：
Scha.
有趣的“变色龙”荧光生物探针用于脂滴-溶酶体相互作用的可视化
影响因子：
14
作者：
Hu, Rong;Chen, Bin;Tang, Ben Zhong
通讯作者：
Tang, Ben Zhong