Comparison of wild rice (Oryza longistaminata) tissues identifies rhizome-specific bacterial and archaeal endophytic microbiomes communities and network structures.
Comparison of wild rice (Oryza longistaminata) tissues identifies rhizome-specific bacterial and archaeal endophytic microbiomes communities and network structures.
复制标题
DOI：
10.1371/journal.pone.0246687
复制
发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Ding X
中科院分区：
文献类型：
--
作者：
Peng X;Xie J;Li W;Xie H;Cai Y;Ding X
与与根相关的生境相比，人们对微生物区系在水稻其他器官中的作用知之甚少，特别是多年生野生稻的根茎，这些信息可能对农业具有重要意义。长粒野生稻是一种多年生野生稻，在贫瘠的土壤上具有生物量大、氮素利用率高、抗病虫害等农艺性状。在这里，我们使用16S rRNA基因测序的方法，将根茎的内生细菌和古生菌群落以及网络结构与长穗野生稻的其他部分进行了比较。不同隔室间的微生物区系差异较大，群落结构也存在较大差异。根茎微生物群落表现出较低的分类多样性和系统发育多样性，且在4个群落中网络复杂性最低。与根和叶相比，根茎的系统发育聚类较少，但与茎的系统发育聚类相似。根茎中的主要属为链球菌属、芽孢杆菌属和甲基乳杆菌属。ASV属于Enhybacter、YS2和Roseburia，专门存在于根茎中。豆科植物在根茎和茎中的相对丰度显著高于叶和根。值得注意的是，在所有的隔室中都观察到了II型甲烷氧化菌，包括优势的甲氧菌科，这可能通过促进在氮素营养不良的条件下依赖CH4的氮气固定而使宿主受益。我们的数据提供了跨越不同隔室的寄主和微生物组相互作用的可靠知识，并为研究长秆野生稻在营养贫乏环境中的适应机制提供了农业生物肥料开发的指导方针。
Compared with root-associated habitats, little is known about the role of microbiota inside other rice organs, especially the rhizome of perennial wild rice, and this information may be of importance for agriculture. Oryza longistaminata is perennial wild rice with various agronomically valuable traits, including large biomass on poor soils, high nitrogen use efficiency, and resistance to insect pests and disease. Here, we compared the endophytic bacterial and archaeal communities and network structures of the rhizome to other compartments of O. longistaminata using 16S rRNA gene sequencing. Diverse microbiota and significant variation in community structure were identified among different compartments of O. longistaminata. The rhizome microbial community showed low taxonomic and phylogenetic diversity as well as the lowest network complexity among four compartments. Rhizomes exhibited less phylogenetic clustering than roots and leaves, but similar phylogenetic clustering with stems. Streptococcus, Bacillus, and Methylobacteriaceae were the major genera in the rhizome. ASVs belonging to the Enhydrobacter, YS2, and Roseburia are specifically present in the rhizome. The relative abundance of Methylobacteriaceae in the rhizome and stem was significantly higher than that in leaf and root. Noteworthy type II methanotrophs were observed across all compartments, including the dominant Methylobacteriaceae, which potentially benefits the host by facilitating CH4-dependent N2 fixation under nitrogen nutrient-poor conditions. Our data offers a robust knowledge of host and microbiome interactions across various compartments and lends guidelines to the investigation of adaptation mechanisms of O. longistaminata in nutrient-poor environments for biofertilizer development in agriculture.
登录
查看更多内容
嗜热甲烷杆菌的比较蛋白质组分析揭示了不同温度条件下 H-2 和 CO2 形成甲烷
影响因子：
3.4
作者：
Liu C;Mao L;Zheng X;Yuan J;Hu B;Cai Y;Xie H;Peng X;Ding X
通讯作者：
Ding X
影响因子：
4.8
作者：
Clark, JS
通讯作者：
Clark, JS
影响因子：
56.9
作者：
Louca, Stilianos;Parfrey, Laura Wegener;Doebeli, Michael
通讯作者：
Doebeli, Michael
影响因子：
2.2
作者：
Ikeda S;Sasaki K;Okubo T;Yamashita A;Terasawa K;Bao Z;Liu D;Watanabe T;Murase J;Asakawa S;Eda S;Mitsui H;Sato T;Minamisawa K
通讯作者：
Minamisawa K
影响因子：
5.6
作者：
Jin, Jie;Long, Weixiong;Li, Shaoqing
通讯作者：
Li, Shaoqing