Characterization of Iflavirus in the Red Flour Beetle, Tribolium castaneum (Coleoptera; Tenebrionidae).
Characterization of Iflavirus in the Red Flour Beetle, Tribolium castaneum (Coleoptera; Tenebrionidae).
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DOI：
10.3390/insects14030220
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发表时间：
2023-02-23
期刊：
影响因子：
3
通讯作者：
Park Y
中科院分区：
文献类型：
--
作者：
Fatehi S;Aikins M;Philips TW;Brown S;Zhu KY;Scully ED;Park Y
赤拟谷盗（Tribolium castaneum）是一种携带正单链RNA病毒赤拟谷盗黄病毒（Tribolium castaneum iflavirus，TcIV）的模式甲虫。这项研究表明，TcIV在不同的实验室培养物、不同的菌株、不同实验室保存的同一菌株以及TcA细胞系中普遍存在。本研究支持经卵巢传播及其在神经系统中的患病率。新的TcIV，显示没有可观察到的致病性，提供了一个机会，研究在这个模型甲虫物种的病毒和免疫系统之间的相互作用。黄病毒是一组主要分布于节肢动物体内的病毒。本研究对赤拟谷盗黄曲霉病毒（TcIV）的不同实验室分离株和GenBank中的序列阅读档案（SRA）进行了调查。TcIV仅对T具有高度特异性。在其他七种拟步甲中没有发现，包括与之密切相关的拟步甲T.弗里曼尼采用Taqman定量PCR技术对50个不同品系的猪链球菌进行检测，发现不同实验室的同一菌株和不同菌株的感染程度存在很大差异。我们发现~63%（43株中的27株）的T.不同实验室中的castaneum菌株对于TcIV PCR是阳性的，具有在7个数量级的范围内的大的变化程度，表明TcIV取决于饲养条件而高度波动。TcIV在神经系统中普遍存在，在性腺和肠道中发现低水平。经卵传播的实验支持与表面消毒鸡蛋。有趣的是，TcIV感染没有表现出可观察到的致病性。TcIV提供了一个机会来研究病毒和这种模式甲虫物种的免疫系统之间的相互作用。
A model beetle, Tribolium castaneum, carries a positive single-stranded RNA virus Tribolium castaneum iflavirus (TcIV). This study shows that the TcIV is prevalent among different laboratory cultures, different strains, and the same strain maintained in different laboratories, and in the Tribolium cell line TcA. Transovarial transmissions and their prevalence in the nervous system were supported in this study. The novel TcIV, showing no observable pathogenicity, offers an opportunity to study the interaction between the virus and the immune system in this model beetle species. Iflavirus is a group of viruses distributed mainly in arthropod species. We surveyed Tribolium castaneum iflavirus (TcIV) in different laboratory strains and in Sequence Read Archives (SRA) in GenBank. TcIV is highly specific to only T. castaneum and is not found in seven other Tenebrionid species, including the closely related species T. freemani. The same strains from different laboratories and different strains displayed largely different degrees of infections in the examination of 50 different lines by using Taqman-based quantitative PCR. We found that ~63% (27 out of 43 strains) of T. castaneum strains in different laboratories are positive for TcIV PCR with large degrees of variation, in the range of seven orders of magnitude, indicating that the TcIV is highly fluctuating depending on the rearing conditions. The TcIV was prevalent in the nervous system with low levels found in the gonad and gut. The transovarial transmission was supported in the experiment with surface-sterilized eggs. Interestingly, TcIV infection did not show observable pathogenicity. TcIV offers an opportunity to study the interaction between the virus and the immune system of this model beetle species.
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