Characterization of Two Novel Insect-Specific Viruses Discovered in the Green Leafhopper, Cicadella viridis.
Characterization of Two Novel Insect-Specific Viruses Discovered in the Green Leafhopper, Cicadella viridis.
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昆虫特异性病毒（ISV）作为一种潜在的生物制剂，已受到越来越多的关注。本研究采用元转录组、RT-PCR和RACE方法，对绿色叶蝉中的两种ISV（Cicadella viridis iflavirus 1，CvIfV 1; Cicadella viridis nido-like virus 1，CvNiLV 1）的全基因组进行了研究。CvIfV 1是黄曲霉病毒属的成员，具有典型的黄曲霉病毒基因组结构。当CvIfV 1攻击宿主昆虫时，引发抗病毒RNA干扰（RNAi），这导致21-nt病毒衍生的siRNA（vsiRNA）的大量积累。CvNiLV 1簇属于巢病毒目中一个独特的未分类的病毒进化枝，与感染脊椎动物宿主的病毒进化相关。CvNiLV 1也被宿主RNAi途径靶向，产生了5′-端具有较强A/U偏好的21-nt vsiRNA。我们的研究提供了有价值的信息，在叶蝉的ISV，并可能被证明是有用的害虫管理。昆虫特异性病毒（ISV）是最有前途的昆虫生物防治剂之一。绿色叶蝉Cicadellaviridis（Linnaeus）是一种重要的农业害虫，对果树、柳树和大田作物造成严重的经济损失。作为大叶蝉科的代表种，中国叶蝉的ISVs。迄今为止，还没有研究过绿球藻。在这项研究中，全基因组序列的两个ISV，命名为Cicadella viridis iflavirus 1（CvIfV 1），和Cicadella viridis nido-like virus 1（CvNiLV 1），使用元转录组的方法。同源性检索和系统发育分析表明，CvIfV 1属于黄病毒科（Iflavirus属）的新成员，具有典型的黄病毒基因组结构，而CvNiLV 1属于巢状病毒目（Nidovirales）的未分类分支/家族。此外，还对病毒来源的小干扰RNA（vsiRNA）进行了分析，以研究C.绿色的vsiRNA具有由宿主siRNA介导的抗病毒免疫产生的典型模式，包括来自正义和反义基因组链的21-nt vsiRNA的偏好，以及病毒基因组5′-末端的强A/U偏好。本研究首次为叶蝉中的ISV提供了有价值的信息，这可能对叶蝉的控制有帮助。绿色未来
Insect-specific viruses (ISVs) have gained increasing attention for their potential use as biological agents. In this study, the full genomes of two ISVs (Cicadella viridis iflavirus 1, CvIfV1; Cicadella viridis nido-like virus 1, CvNiLV1) were revealed in green leafhoppers, using metatranscriptome, RT-PCR, and RACE approaches, respectively. CvIfV1 is a member of iflavirus and has a typical iflavirus genome organization. An antiviral RNA interference (RNAi) was triggered when host insects were challenged with CvIfV1, which resulted in an abundant accumulation of 21-nt virus-derived siRNAs (vsiRNAs). CvNiLV1 clusters are within a distinct unclassified clade of viruses in the order Nidovirales, and evolutionary related with viruses that infect vertebrate hosts. CvNiLV1 was also targeted by the host antiviral RNAi pathway, and generated the 21-nt vsiRNAs with a strong A/U bias in the 5′-terminal. Our study provided valuable information on ISVs in leafhoppers, and might prove useful in pest management. Insect-specific viruses (ISV) are one of the most promising agents for the biological control of insects. The green leafhopper, Cicadella viridis (Linnaeus), is an significant pest in agriculture, and causes economic losses to fruit trees, willows, and field crops. As a representative species of the large family Cicadellidae, ISVs in C. viridis have never been studied, to date. In this study, the full genome sequences of two ISVs, named Cicadella viridis iflavirus 1 (CvIfV1), and Cicadella viridis nido-like virus 1 (CvNiLV1), were revealed using a metatranscriptomic approach. A homology search and phylogenetic analysis indicated that CvIfV1 is a new member in the family Iflaviridae (genus Iflavirus) with a typical iflavirus genome organization, whereas CvNiLV1 belongs to the unclassified clade/family of the order Nidovirales. In addition, analysis of virus-derived small interfering RNAs (vsiRNAs) was performed to investigate the antiviral RNA interference (RNAi) response of C. viridis. The vsiRNAs exhibit typical patterns produced by host siRNA-mediated antiviral immunity, including a preference of 21-nt vsiRNAs derived equally from the sense and antisense genomic strands, and a strong A/U bias in the 5′-terminus of the viral genomes. Our study provides valuable information for ISVs in leafhoppers for the first time, which might prove useful in the control of C. viridis in future.
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DOI：
10.1016/j.bbrc.2020.11.015
发表时间：
2021-01-29
影响因子：
3.1
作者：
Gulyaeva AA;Gorbalenya AE
通讯作者：
Gorbalenya AE
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5
作者：
Gorbalenya AE;Enjuanes L;Ziebuhr J;Snijder EJ
通讯作者：
Snijder EJ
影响因子：
10.7
作者：
Darriba, Diego;Posada, David;Flouri, Tomas
通讯作者：
Flouri, Tomas
影响因子：
5.8
作者：
Geoghegan JL;Holmes EC
通讯作者：
Holmes EC