Bisphenol-A exposure alters endometrial progesterone receptor expression in the nonhuman primate.
Bisphenol-A exposure alters endometrial progesterone receptor expression in the nonhuman primate.
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DOI：
10.1016/j.fertnstert.2011.04.010
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发表时间：
2011-07
影响因子：
6.7
通讯作者：
Taylor, Hugh S.
中科院分区：
文献类型：
--
作者：
Aldad, Tamir S.;Rahmani, Nora;Leranth, Csaba;Taylor, Hugh S.
关键词：
探讨BPA对非人灵长类动物和人细胞子宫内膜PR表达的影响。双酚a是一种雌激素内分泌干扰物。BPA暴露和孕酮作用减弱都与流产、子宫内膜异位症和子宫内膜增生/癌症有关。灵长类动物的对照试验。切除卵巢后给予双酚a （50μg/kg/d）、雌二醇、两者均给予或对照。雌二醇和双酚a用于石川细胞。利用免疫组化和qPCR表达PR。与对照组相比，雌二醇处理的灵长类动物PR表达增加。与雌二醇单独暴露相比，雌二醇和BPA联合暴露导致PR表达降低（p<0.01）。在雌二醇处理的石川细胞中，PR的表达增加了5.1倍，而在雌二醇和BPA同时处理的石川细胞中，PR的表达减少到雌二醇单独处理的0.6倍（p<0.05）。单是BPA就有弱雌激素的作用。然而，当与雌二醇一起使用时，BPA会减少雌二醇诱导的PR表达。据报道，BPA在人体中的雌激素样效应可能是通过PR阻断介导的，从而导致通常由黄体酮引起的雌激素抑制作用减弱。PR表达的减少可能是先前有关BPA暴露与人类子宫内膜功能障碍的报道的基础。
To evaluate the effect of BPA on endometrial PR expression in non-human primates and human cells. BPA is a xenoestrogen endocrine disruptor. Both BPA exposure and diminished progesterone action have been associated with pregnancy loss, endometriosis and endometrial hyperplasia/cancer. Controlled trial in primates. University African green monkeys After oophorectomy, BPA (50μg/kg/day), estradiol, both or vehicle control were administered. . Estradiol and BPA were used in Ishikawa cells. PR expression using IHC and qPCR. PR expression was increased in estradiol treated primates compared to controls. Exposure to the combination of estradiol and BPA resulted in decreased PR expression compared to estradiol exposure alone (p<0.01). In Ishikawa cells treated with estradiol, PR expression increased 5.1 fold, however, when Ishikawa cells were simultaneously treated with estradiol and BPA, PR expression was decreased to 0.6 fold that of cells treated with estradiol alone (p<0.05). BPA alone functions as a weak estrogen. However, when administered with estradiol, BPA diminishes estradiol induced PR expression. The estrogen-like effect of BPA reported in exposed humans may be mediated by PR blockade and a resultant decrease in the estrogen inhibition normally imparted by progesterone. Diminished PR expression may underlie previous reports linking BPA exposure to endometrial dysfunction in humans.
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影响因子：
3.3
作者：
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