454 pyrosequencing analysis on faecal samples from a randomized DBPC trial of colicky infants treated with Lactobacillus reuteri DSM 17938.
454 pyrosequencing analysis on faecal samples from a randomized DBPC trial of colicky infants treated with Lactobacillus reuteri DSM 17938.
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DOI：
10.1371/journal.pone.0056710
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Savino F
中科院分区：
文献类型：
--
作者：
Roos S;Dicksved J;Tarasco V;Locatelli E;Ricceri F;Grandin U;Savino F
采用16 S rRNA基因的大规模DNA测序技术，分析给予L。reuteri DSM 17938或安慰剂。29名绞痛婴儿（年龄10-60天）入选，随机分配接受罗伊氏乳杆菌（108 cfu）或安慰剂，每天一次，持续21天。应答者定义为第21天与起始点相比每日哭泣时间减少50%的受试者。使用454焦磷酸测序分析来自第1天和第21天的粪便样品的微生物群。引物：使用补充有454个衔接子和样品特异性条形码的Bakt_341F和Bakt_805R进行16 S rRNA基因的PCR扩增。通过排列多变量方差分析探索数据的结构，并通过排序分析可视化不同变量的影响。婴儿粪便菌群主要由变形菌门、厚壁菌门、放线菌门和拟杆菌门组成。患有绞痛的婴儿的微生物群的组成具有非常高的个体间变异性，厚壁菌门/拟杆菌门的比例从4000到0.025不等。然而，在个体的基础上，微生物群随着时间的推移相对稳定。治疗L. reuteri DSM 17938没有改变微生物群的总体组成，但是当比较应答者与非应答者时，与第0天相比，应答者组在第21天具有增加的拟杆菌门和拟杆菌属的相对丰度。此外，门组成的婴儿在21日龄可以分为三个肠型组，占主导地位的厚壁菌门，拟杆菌门，放线菌，分别。 L. reuteri DSM 17938不影响微生物群的总体组成。然而，应答婴儿中拟杆菌的增加表明，绞痛症状的减少与微生物群的变化有关。ClinicalTrials.gov NCT00893711
To analyze the global microbial composition, using large-scale DNA sequencing of 16 S rRNA genes, in faecal samples from colicky infants given L. reuteri DSM 17938 or placebo. Twenty-nine colicky infants (age 10–60 days) were enrolled and randomly assigned to receive either Lactobacillus reuteri (108 cfu) or a placebo once daily for 21 days. Responders were defined as subjects with a decrease of 50% in daily crying time at day 21 compared with the starting point. The microbiota of faecal samples from day 1 and 21 were analyzed using 454 pyrosequencing. The primers: Bakt_341F and Bakt_805R, complemented with 454 adapters and sample specific barcodes were used for PCR amplification of the 16 S rRNA genes. The structure of the data was explored by using permutational multivariate analysis of variance and effects of different variables were visualized with ordination analysis. The infants’ faecal microbiota were composed of Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes as the four main phyla. The composition of the microbiota in infants with colic had very high inter-individual variability with Firmicutes/Bacteroidetes ratios varying from 4000 to 0.025. On an individual basis, the microbiota was, however, relatively stable over time. Treatment with L. reuteri DSM 17938 did not change the global composition of the microbiota, but when comparing responders with non-responders the group responders had an increased relative abundance of the phyla Bacteroidetes and genus Bacteroides at day 21 compared with day 0. Furthermore, the phyla composition of the infants at day 21 could be divided into three enterotype groups, dominated by Firmicutes, Bacteroidetes, and Actinobacteria, respectively. L. reuteri DSM 17938 did not affect the global composition of the microbiota. However, the increase of Bacteroidetes in the responder infants indicated that a decrease in colicky symptoms was linked to changes of the microbiota. ClinicalTrials.gov NCT00893711
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
14.2
作者：
Adlerberth, Ingegerd;Strachan, David P.;Wold, Agnes E.
通讯作者：
Wold, Agnes E.
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
5.1
作者：
Rhoads, J. Marc;Fatheree, Nicole Y.;Ferris, Michael J.
通讯作者：
Ferris, Michael J.