Cyanobacterial cell lineage analysis of the spatiotemporal hetR expression profile during heterocyst pattern formation in Anabaena sp. PCC 7120.
Cyanobacterial cell lineage analysis of the spatiotemporal hetR expression profile during heterocyst pattern formation in Anabaena sp. PCC 7120.
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DOI：
10.1371/journal.pone.0007371
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发表时间：
2009-10-12
期刊：
影响因子：
3.7
通讯作者：
Iwasaki H
中科院分区：
文献类型：
--
作者：
Asai H;Iwamori S;Kawai K;Ehira S;Ishihara J;Aihara K;Shoji S;Iwasaki H
在丝状蓝藻鱼腥藻7120中，固氮异形胞的形成是已知细胞分化中最简单的模式形成之一。大多数以前的研究异形胞图案是基于统计分析，使用收集或观察到的细胞在不同的时间从液体培养，这将掩盖随机波动影响的过程中的模式形成动力学在一个单一的细菌细丝。为了分析在单丝水平的异形胞形成的时空动态，在这里，我们开发了一个培养系统，同时监测细菌的发展，基因表达，和藻胆体荧光。我们还开发了微液室阵列，以同时分析多个鱼腥藻丝。细胞谱系分析表明，初始分布的hetR：：gfp和藻胆体荧光信号在氮降压与所产生的分布发达的异形胞不相关。延时观察还揭示了一个动态的hetR表达谱在单丝水平，包括瞬时上调伴随细胞分裂，这并不总是导致异形胞的发展。此外，一些细胞分化成异形胞后，氮降压没有细胞分裂，表明在母细胞中的细胞分裂不是异形胞分化的必要条件。
Diazotrophic heterocyst formation in the filamentous cyanobacterium, Anabaena sp. PCC 7120, is one of the simplest pattern formations known to occur in cell differentiation. Most previous studies on heterocyst patterning were based on statistical analysis using cells collected or observed at different times from a liquid culture, which would mask stochastic fluctuations affecting the process of pattern formation dynamics in a single bacterial filament. In order to analyze the spatiotemporal dynamics of heterocyst formation at the single filament level, here we developed a culture system to monitor simultaneously bacterial development, gene expression, and phycobilisome fluorescence. We also developed micro-liquid chamber arrays to analyze multiple Anabaena filaments at the same time. Cell lineage analyses demonstrated that the initial distributions of hetR::gfp and phycobilisome fluorescence signals at nitrogen step-down were not correlated with the resulting distribution of developed heterocysts. Time-lapse observations also revealed a dynamic hetR expression profile at the single-filament level, including transient upregulation accompanying cell division, which did not always lead to heterocyst development. In addition, some cells differentiated into heterocysts without cell division after nitrogen step-down, suggesting that cell division in the mother cells is not an essential requirement for heterocyst differentiation.
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影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S
影响因子：
2.7
作者：
Allard, Jun F.;Hill, Alison L.;Rutenberg, Andrew D.
通讯作者：
Rutenberg, Andrew D.
影响因子：
3.2
作者：
Aldea, M. Ramona;Mella-Herrera, Rodrigo A.;Golden, James W.
通讯作者：
Golden, James W.
DOI：
10.1099/00221287-96-1-175
发表时间：
1976-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
BRADLEY, S;CARR, NG
通讯作者：
CARR, NG
影响因子：
56.9
作者：
Yoon, HS;Golden, JW
通讯作者：
Golden, JW