Comparisons of oral, intestinal, and pancreatic bacterial microbiomes in patients with pancreatic cancer and other gastrointestinal diseases.
Comparisons of oral, intestinal, and pancreatic bacterial microbiomes in patients with pancreatic cancer and other gastrointestinal diseases.
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DOI：
10.1080/20002297.2021.1887680
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发表时间：
2021-02-14
影响因子：
4.5
通讯作者：
Michaud DS
中科院分区：
文献类型：
--
作者：
Chung M;Zhao N;Meier R;Koestler DC;Wu G;de Castillo E;Paster BJ;Charpentier K;Izard J;Kelsey KT;Michaud DS
背景：口腔微生物群被认为在包括癌症在内的全身性疾病中起重要作用。研究方法：我们收集了52名受试者的口腔样本（舌、颊、龈上和唾液）和胰腺组织或肠道样本，并使用高通量DNA测序表征了16 S rRNA基因。结果如下：Bray-Curtis图显示口腔中的细菌群落与肠和胰腺组织样品中的细菌群落之间存在明显的分离。PERMANOVA试验表明，口腔样本的细菌群落与龈上和唾液样本相似，胰管样本与胰腺肿瘤样本相似，但所有其他样本彼此之间存在显著差异。在口腔和胰腺或肠样品之间共有总共73个独特的扩增子序列变体（ASV）。只有四个ASV显示出显著的一致性，和两个特定的细菌物种（麻疹双胞菌和具核梭杆菌亚种）。vincentii）在调整受试者内相关性和疾病状态后，在口腔和肠或胰腺样品之间显示出一致的存在或不存在模式。最后，微生物共丰度分析显示，口腔、唾液、十二指肠、空肠和胰腺肿瘤样品中的微生物组成员之间存在不同的菌株水平聚类模式。结论：我们的研究结果表明，在胰腺癌和其他胃肠道疾病患者中，口腔、肠道和胰腺细菌微生物组重叠，但表现出不同的共丰度模式。
Background: Oral microbiota is believed to play important roles in systemic diseases, including cancer. Methods: We collected oral samples (tongue, buccal, supragingival, and saliva) and pancreatic tissue or intestinal samples from 52 subjects, and characterized 16S rRNA genes using high-throughput DNA sequencing. Results: Bray–Curtis plot showed clear separations between bacterial communities in the oral cavity and those in intestinal and pancreatic tissue samples. PERMANOVA tests indicated that bacterial communities from buccal samples were similar to supragingival and saliva samples, and pancreatic duct samples were similar to pancreatic tumor samples, but all other samples were significantly different from each other. A total of 73 unique Amplicon Sequence Variants (ASVs) were shared between oral and pancreatic or intestinal samples. Only four ASVs showed significant concordance, and two specific bacterial species (Gemella morbillorum and Fusobacterium nucleatum subsp. vincentii) showed consistent presence or absence patterns between oral and intestinal or pancreatic samples, after adjusting for within-subject correlation and disease status. Lastly, microbial co-abundance analyses showed distinct strain-level cluster patterns among microbiome members in buccal, saliva, duodenum, jejunum, and pancreatic tumor samples. Conclusions: Our findings indicate that oral, intestinal, and pancreatic bacterial microbiomes overlap but exhibit distinct co-abundance patterns in patients with pancreatic cancer and other gastrointestinal diseases.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
影响因子：
6.4
作者：
Bokulich NA;Dillon MR;Zhang Y;Rideout JR;Bolyen E;Li H;Albert PS;Caporaso JG
通讯作者：
Caporaso JG
影响因子：
64.8
作者：
Claesson, Marcus J.;Jeffery, Ian B.;O'Toole, Paul W.
通讯作者：
O'Toole, Paul W.