Influence of urban landscapes on population dynamics in a short-distance migrant mosquito: evidence for the dengue vector Aedes aegypti.
Influence of urban landscapes on population dynamics in a short-distance migrant mosquito: evidence for the dengue vector Aedes aegypti.
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DOI：
10.1371/journal.pntd.0000634
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发表时间：
2010-03-16
影响因子：
3.8
通讯作者：
Severson DW
中科院分区：
文献类型：
--
作者：
Hemme RR;Thomas CL;Chadee DD;Severson DW
登革热病毒在大多数热带和亚热带地区流行。由于没有经过验证的疫苗，登革热预防主要是通过控制蚊子媒介埃及伊蚊来完成的。虽然通常认为扩散距离为~ 100米，但由于景观特征作为扩散的屏障或走廊，城市地区的扩散模式可能会有所不同。人为特征最终影响影响媒介能力和杀虫剂抗性的基因流动。因此，深入了解影响扩散的参数对于有效实施任何蚊子种群抑制计划至关重要。目前正在考虑的种群替换和遗传控制策略也取决于对城市环境中蚊子扩散的彻底了解。我们研究了一条主要高速公路在2年期间对扩散模式的影响。在西印度群岛特立尼达Charlieville附近的乌利亚巴特勒公路（UBH）东侧和西侧采集埃及伊蚊幼虫，研究UBH对所观察到的种群结构的影响。利用9颗微卫星、2个snp和710 bp的mtDNA细胞色素氧化酶亚基1 （CO1）序列对样品进行分子分析。鉴定出三个CO1单倍型，其中一个仅在2006年和2007年在道路东侧发现。使用mtCO1和核标记的AMOVA显示了东部和西部收集之间的显著差异。我们的研究结果表明，城市环境中存在埃及伊蚊传播的人为障碍，在登革热暴发期间实施控制计划和种群抑制或替代计划时应考虑这些障碍。全世界有25亿人面临登革热感染的风险，但没有疫苗或治疗方法。因此，登革热的预防主要集中在控制其媒介蚊子埃及伊蚊上。传统的蚊虫控制方法通常包括使用杀虫剂和减少孳生场所的源头。目前，人们正在考虑采用昆虫不育技术和用无登革热能力的转基因蚊子替代蚊虫等新的登革热控制措施。所有人口控制计划的成功部分取决于对蚊子种群生态学的理解，包括人类对城市景观的影响如何影响蚊子的扩散和扩张。我们进行了一项为期两年的种群遗传研究，研究西印度群岛特立尼达的主要大都市高速公路如何影响蚊子的传播。正如核和线粒体DNA序列的显著分化所证明的那样，高速公路是扩散的重要障碍。我们的研究结果表明，通过在已知的限制种群扩散的景观屏障上划定蚊虫控制区域，可以有效地利用人为景观特征来加强种群抑制/替代措施。
Dengue viruses are endemic across most tropical and subtropical regions. Because no proven vaccines are available, dengue prevention is primarily accomplished through controlling the mosquito vector Aedes aegypti. While dispersal distance is generally believed to be ∼100 m, patterns of dispersion may vary in urban areas due to landscape features acting as barriers or corridors to dispersal. Anthropogenic features ultimately affect the flow of genes affecting vector competence and insecticide resistance. Therefore, a thorough understanding of what parameters impact dispersal is essential for efficient implementation of any mosquito population suppression program. Population replacement and genetic control strategies currently under consideration are also dependent upon a thorough understanding of mosquito dispersal in urban settings. We examined the effect of a major highway on dispersal patterns over a 2 year period. A. aegypti larvae were collected on the east and west sides of Uriah Butler Highway (UBH) to examine any effect UBH may have on the observed population structure in the Charlieville neighborhood in Trinidad, West Indies. A panel of nine microsatellites, two SNPs and a 710 bp sequence of mtDNA cytochrome oxidase subunit 1 (CO1) were used for the molecular analyses of the samples. Three CO1 haplotypes were identified, one of which was only found on the east side of the road in 2006 and 2007. AMOVA using mtCO1 and nuclear markers revealed significant differentiation between the east- and west-side collections. Our results indicate that anthropogenic barriers to A. aegypti dispersal exist in urban environments and should be considered when implementing control programs during dengue outbreaks and population suppression or replacement programs. Worldwide, 2.5 billion people are at risk for dengue infection, with no vaccine or treatment available. Thus dengue prevention is largely focused on controlling its mosquito vector, Aedes aegypti. Traditional mosquito control approaches typically include insecticide applications and breeding site source reduction. Presently, novel dengue control measures including the sterile insect technique and population replacement with dengue-incompetent transgenic mosquitoes are also being considered. Success of all population control programs is in part dependent upon understanding mosquito population ecology, including how anthropogenic effects on the urban landscape influence dispersal and expansion. We conducted a two year population genetic study examining how a major metropolitan highway impacts mosquito dispersal in Trinidad, West Indies. As evidenced by significant differentiation using both nuclear and mitochondrial DNA sequences, the highway acted as a significant barrier to dispersal. Our results suggest that anthropogenic landscape features can be used effectively to enhance population suppression/replacement measures by defining mosquito control zones along recognized landscape barriers that limit population dispersal.
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影响因子：
1.9
作者：
Colton, YM;Chadee, DD;Severson, DW
通讯作者：
Severson, DW
影响因子：
5.9
作者：
Bhattacharya, M;Primack, RB;Gerwein, J
通讯作者：
Gerwein, J
影响因子：
2.7
作者：
Chadee, Dave D.;Doon, Rohit;Severson, David W.
通讯作者：
Severson, David W.
影响因子：
2.6
作者：
Excoffier, Laurent;Laval, Guillaume;Schneider, Stefan
通讯作者：
Schneider, Stefan
DOI：
10.4269/ajtmh.2000.62.200
发表时间：
2000-02-01
影响因子：
3.3
作者：
Gorrochotegui-Escalante, N;Munoz, MD;Black, WC
通讯作者：
Black, WC