Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2/M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways.
Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2/M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways.
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Arca subcrenata 的多肽组分通过 ROS 介导的 MAPK 途径诱导 HeLa 细胞凋亡和 G2/M 期停滞
DOI：
10.1155/2015/930249
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Yu R
中科院分区：
文献类型：
--
作者：
Hu X;Zhang Z;Liu T;Song L;Zhu J;Guo Z;Cai J;Yu R
Arca subcrenata is documented in the literature of marine Traditional Chinese Medicine. Polypeptide fraction from A. subcrenata, coded as P2, was demonstrated to possess significant antitumor activity in our previous study. However, the underlying mechanism remains undefined. The present study was carried out to investigate the underlying antitumor mechanism of P2 in human cervical cancer HeLa cells by MTT, FCM, LSCM, and western blot assays. The results revealed that P2 significantly induced apoptosis of HeLa cells in a concentration- and time-dependent manner. High level of ROS was provoked by P2, which was in turn responsible for induction of apoptosis through activation of intrinsic mitochondrial pathway and JNK1/2, p38 MAPK pathways, as well as inhibition of ERK1/2 pathway, as evidenced by the abrogation of P2's effect on HeLa cells preincubated with the ROS scavenger NAC. P2 also was observed to display significant effect on G2/M phase arrest by downregulating the expression of cyclin B1/cdc2 complex and upregulating the expression of p21. These findings demonstrate that P2 induces apoptosis and G2/M phase arrest in HeLa cells through ROS-mediated MAPKs pathways, suggesting that P2 would be worth investigating as a promising agent within the scope of marine drugs for treatment of cervical cancer.
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影响因子：
5.4
作者：
Silva Costa L;Silva Telles CB;Medeiros Oliveira R;Duarte Barreto Nobre LT;Dantas-Santos N;Barros Gomes Camara R;Santana Santos Pereira Costa M;Almeida-Lima J;Melo-Silveira RF;Lopes Albuquerque IR;Leite EL;Oliveira Rocha HA
通讯作者：
Oliveira Rocha HA
影响因子：
9.7
作者：
Lee, Won-Hee;Choi, Joon-Seok;Surh, Young-Joon
通讯作者：
Surh, Young-Joon
影响因子：
9.7
作者：
Moon, Dong-Oh;Kim, Mun-Ock;Kim, Gi-Young
通讯作者：
Kim, Gi-Young
影响因子：
64.8
作者：
Chang, LF;Karin, M
通讯作者：
Karin, M
影响因子：
64.5
作者：
ELLIS, HM;HORVITZ, HR
通讯作者：
HORVITZ, HR