The characteristic expression pattern of BMI-1 and SALL4 genes in placenta tissue and cord blood.
The characteristic expression pattern of BMI-1 and SALL4 genes in placenta tissue and cord blood.
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DOI：
10.1186/scrt199
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发表时间：
2013-04-30
影响因子：
7.5
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Chen S;Liu S;Xu L;Yang L;Jin Z;Ma Y;Li B;Wu X;Yang J;Ma Y;Li Y
SALL 4和BMI-1是造血的重要因子。胎盘组织（PT）和脐带血（CB）富含造血干/祖细胞（HSC/HPC），但其SALL 4和BMI-1表达水平仍未知。采用实时荧光定量PCR方法检测10例PT和CB中上述基因的表达水平，并以10例健康献血员作为对照。BMI-1和SALL 4基因表达水平在PT组中显著增高，（中位数：分别为17.548和34.362）比脐带血单个核细胞（CBMCs）（中位数：分别为2.071和11.300）（P = 0.0001和P = 0.007）和健康外周血单核细胞（PBMC）（中位数分别为0.259和0.384）（P = 0.001和P <0.0001），PBMCs中的表达水平低于CBMCs（P = 0.029和P = 0.002）。BMI-1与SALL 4基因在PT组和CB组中呈正相关，而在健康组中相关性不显著。PT和CB中各基因的表达水平之间也无显著相关性。这些结果描述了胎盘组织和脐带血中BMI-1和SALL 4基因表达模式的特征。具有较高表达水平的两个基因的胎盘组织可以被认为是用于SALL 4相关HPC扩增的潜在资源。
SALL4 and BMI-1 are important factors in hematopoiesis. Placental tissue (PT) and umbilical cord blood (CB) are rich in hematopoietic stem/progenitor cells (HSCs/HPCs), but their SALL4 and BMI-1 expression levels remain unknown. Real-time PCR was used to determine the expression level of these genes in PT and CB from ten cases, and ten healthy donors were used as controls. A significantly higher BMI-1 and SALL4 gene expression level was found in PT (median: 17.548 and 34.362, respectively) than in cord blood mononuclear cells (CBMCs) (median: 2.071 and 11.300, respectively) (P = 0.0001 and P = 0.007) and healthy peripheral blood mononuclear cells (PBMCs) (median: 0.259 and 0.384, respectively) (P = 0.001 and P <0.0001), and their expression level was lower in PBMCs than in CBMCs (P = 0.029 and P = 0.002). A positive correlation between the BMI-1 and SALL4 genes was found in the PT and CB groups, while there was no significant correlation between these genes in the healthy group. There was also no significant correlation between the expression level of each gene in PT and CB. These results describe the characteristic features of the BMI-1 and SALL4 gene expression pattern in placental tissue and cord blood. Placental tissue with higher expression level of both genes may be considered as a potential resource for SALL4-related HPC expansion.
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