Sterol composition in plants is specific to pollen, leaf, pollination and pollinator.
Sterol composition in plants is specific to pollen, leaf, pollination and pollinator.
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DOI：
10.1016/j.phytochem.2023.113800
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发表时间：
2023-10
期刊：
影响因子：
3.8
通讯作者：
Stevenson, Philip C.
中科院分区：
文献类型：
--
作者：
Furse, Samuel;Martel, Carlos;Yusuf, Abdikarim;Shearman, Gemma C.;Koch, Hauke;Stevenson, Philip C.
甾醇在植物中有多种作用，包括作为膜成分。固醇也是昆虫必需的营养物质。基于这一点，以及叶片和花粉的不同功能，我们验证了以下假设：(a)同一植物的叶片和花粉的固醇素不同，(b)风媒和虫媒植物的花粉含有不同的固醇，以及(c)赏花粉被子植物的固醇供应与赏花蜜植物不同。研究了一种新的甾醇组学方法，利用LCMS测定了36种不同植物叶片和花粉的甾醇谱。21种甾醇被明确地鉴定出来，还有几种被鉴定出微量。C29甾醇在大多数植物中占主导地位。叶片和花粉中甾醇的组成差异显著，主要甾醇的进化方式也不同。风媒和动物传粉的花粉甾体素也有显著差异，但在花蜜和花粉奖励物种之间没有显著差异。我们的研究结果表明，不同植物组织中的甾醇成分与其生物学功能有关。花粉中的甾醇成分可能是由物理作用而不是传粉昆虫的营养需求驱动的。对不同种类植物组织的甾体素进行了分析。植物体内的甾醇代谢主要集中在C29和C30甾醇上。植物的叶子和花粉的固醇素不同。风媒和动物传粉的花粉甾体素不同。没有证据表明固醇代谢已经进化到支持昆虫传粉媒介。
Sterols have several roles in planta, including as membrane components. Sterols are also essential nutrients for insects. Based on this, and the different functions of leaves and pollen, we tested the hypotheses that (a) the sterolome is different in leaves and pollen from the same plant, (b) pollens from wind- and insect pollinated plants comprise different sterols, and (c) sterol provision in pollen-rewarding angiosperms differs from nectar-rewarding species. A novel approach to sterolomics was developed, using LCMS to determine the sterol profile of leaf and pollen from a taxonomically diverse range of 36 plant species. Twenty-one sterols were identified unambiguously, with several more identified in trace amounts. C29 sterols dominated the sterolome in most plants. The sterol composition was significantly different in leaf and pollen and their main sterols evolved in different ways. The sterolome of pollen from animal- and wind-pollinated was also significantly different, but not between nectar- and pollen-rewarding species. Our results suggest that the sterol composition in different plant tissues is linked to their biological functions. Sterol composition in pollen might be driven by physical role rather than the nutrient needs of pollinating insects. The sterolome of tissues from a taxonomically diverse range of plants were profiled. Sterol metabolism in plants is generally focused on C29 and C30 sterols. The sterolome of leaves and pollen differ within plants. The sterolome of pollens from wind- and animal-pollinated species differ. No evidence that sterol metabolism has evolved to support insect pollinators.
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