Expression of NF-κB p65 phosphorylated at serine-536 in rectal cancer with or without preoperative radiotherapy.
Expression of NF-κB p65 phosphorylated at serine-536 in rectal cancer with or without preoperative radiotherapy.
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DOI：
10.2478/v10019-011-0030-7
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发表时间：
2011-12
影响因子：
2.4
通讯作者：
Sun XF
中科院分区：
文献类型：
--
作者：
Lewander A;Gao J;Adell G;Zhang H;Sun XF
在本研究中，我们研究了直肠癌组织中丝氨酸-536磷酸化的核因子-丝氨酸B-p65(κ-536-p65)及其与术前放疗、临床病理变量和生物学因素的关系。应用免疫组织化学方法检测了141例原发直肠癌、149例正常粘膜和48例淋巴结转移癌组织中P-Ser536-p65的表达。从正常粘膜到原发肿瘤，胞浆中磷酸化Ser536-p65的表达逐渐增加(P<；0.0001，无论是接受放疗的组还是未接受放疗的组)。在从原发肿瘤到转移的两组中，其表达均没有进一步增加(p>；0.05)。P65蛋白表达与肿瘤内皮标记物1(TEM1，p=0.02)、FXYD-3(p=0.001)、再生肝磷酸酶(PRL，p=0.02)、p73(p=0.048)和脑膜瘤相关蛋白(MAC30，p=0.05)的表达呈正相关或趋向相关，而未接受放疗组则无相关关系(p>；0.05)。P-Ser536-p65的表达与包括生存期在内的临床病理因素无关(p>；0.05)。P-Ser536-P65的高表达可能参与了直肠癌的发生发展。放疗后，P-Ser536-P65似乎与生物学因素呈正相关，与肿瘤的恶性程度相关。然而，磷酸化的Ser536-p65与复发和生存期的RT疗效无直接关系。
In the present study, we investigated NF-κB p65 phosphorylated at Serine-536 (phosphor-Ser536-p65) in rectal cancer and its relationship to preoperative radiotherapy (RT), clinicopathological variables and biological factors. Expression of phosphor-Ser536-p65 was examined by using immunohistochemistry in 141 primary rectal cancers, 149 normal mucosa specimens and 48 metastases in the lymph nodes, from rectal cancer patients who participated in a Swedish clinical trial of preoperative RT. The expression of phosphor-Ser536-p65 in the cytoplasm increased from normal mucosa to primary tumour (p<0.0001, for both the group that did and the group that did not received RT). The expression did not further increase from primary tumour to metastasis in either group (p>0.05). Expression of phosphor-Ser536-p65 was positively related to, or tended to be related to, the expression of tumour endothelium marker 1 (TEM1, p=0.02), FXYD-3 (p=0.001), phosphatase of regenerating liver (PRL, p=0.02), p73 (p=0.048) and meningioma associated protein (MAC30, p=0.05) in the group that received RT but there were no such relationships in the group that did not received RT (p>0.05). The expression of phosphor-Ser536-p65 was not related to clinicopathological factors including survival (p>0.05). The increased expression of phosphor-Ser536-p65 may be involved in rectal cancer development. After RT, phosphor-Ser536-p65 seems to be positively related to the biological factors, which associated with more malignant features of tumours. However, phosphor-Ser536-p65 was not directly related to the response of RT based on recurrence and survival.
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DOI：
10.1016/j.ijrobp.2005.12.043
发表时间：
2006-06-01
影响因子：
7
作者：
Wallin, Asa R.;Svanvik, Joar;Sun, Xiao-Feng
通讯作者：
Sun, Xiao-Feng
DOI：
10.1073/pnas.0705647104
发表时间：
2007-11-13
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
4
作者：
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Moynagh, Paul N