Aneuploidy underlies rapid adaptive evolution of yeast cells deprived of a conserved cytokinesis motor.
Aneuploidy underlies rapid adaptive evolution of yeast cells deprived of a conserved cytokinesis motor.
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DOI：
10.1016/j.cell.2008.09.039
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发表时间：
2008-11-28
期刊：
影响因子：
64.5
通讯作者：
Li R
中科院分区：
文献类型：
--
作者：
Rancati G;Pavelka N;Fleharty B;Noll A;Trimble R;Walton K;Perera A;Staehling-Hampton K;Seidel CW;Li R
进化能力是生物系统的基本特征，但这种能力背后的机制和保守核心过程的进化动力学仍然是难以捉摸的。我们在这里表明，酵母细胞删除MYO1，编码唯一的肌球蛋白- ii通常需要细胞质分裂，迅速进化出不同的途径来恢复生长和细胞质分裂。进化的细胞分裂表型与转录组的特定变化相关。多倍体和非整倍体在进化最好的菌株中是常见的遗传改变，非整倍体可以解释基因表达的变化，其水平既与染色体化学计量相关，也远远超出了染色体化学计量。非整倍性的表型效应可以通过myo1Δ细胞中特定调控基因拷贝数的增加来概括。这些结果表明，即使是一个保守的过程也具有进化性，并表明当细胞分裂机制受到强烈干扰时，染色体化学计量学的变化提供了遗传变异的来源，驱动适应性表型的出现。
The ability to evolve is a fundamental feature of biological systems, but the mechanisms underlying this capacity and the evolutionary dynamics of conserved core processes remain elusive. We show here that yeast cells deleted of MYO1, encoding the only myosin-II normally required for cytokinesis, rapidly evolved divergent pathways to restore growth and cytokinesis. The evolved cytokinesis phenotypes correlated with specific changes in the transcriptome. Polyploidy and aneuploidy were common genetic alterations in the best evolved strains, and aneuploidy could account for gene expression changes at levels both correlated with and well beyond chromosome stoichiometry. The phenotypic effect of aneuploidy could be recapitulated with increased copy numbers of specific regulatory genes in myo1Δ cells. These results demonstrate the evolvability of even a well-conserved process and suggest that changes in chromosome stoichiometry provide a source of heritable variation driving the emergence of adaptive phenotypes when the cell division machinery is strongly perturbed.
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影响因子：
7
作者：
Ng, PC;Henikoff, S
通讯作者：
Henikoff, S
影响因子：
64.8
作者：
Aury, Jean-Marc;Jaillon, Olivier;Wincker, Patrick
通讯作者：
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影响因子：
10.7
作者：
Brunet, Frederic G.;Roest Crollius, Hugues;Robinson-Rechavi, Marc
通讯作者：
Robinson-Rechavi, Marc
DOI：
10.1073/pnas.252626899
发表时间：
2002-12-10
影响因子：
11.1
作者：
Ohta, T
通讯作者：
Ohta, T
影响因子：
64.5
作者：
MURRAY, AW;SCHULTES, NP;SZOSTAK, JW
通讯作者：
SZOSTAK, JW