Extracellular vesicles released by fibroblasts undergoing H-Ras induced senescence show changes in lipid profile.
Extracellular vesicles released by fibroblasts undergoing H-Ras induced senescence show changes in lipid profile.
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DOI：
10.1371/journal.pone.0188840
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Emiliani C
中科院分区：
文献类型：
--
作者：
Buratta S;Urbanelli L;Sagini K;Giovagnoli S;Caponi S;Fioretto D;Mitro N;Caruso D;Emiliani C
细胞在其环境中释放细胞外囊泡 (EV)，细胞脂质在其形成、分泌和摄取中发挥重要作用。此外，还有证据表明 EV 转移的脂质对受体的细胞信号传导产生影响。细胞衰老的特征是永久性增殖停滞状态，是肿瘤病变发展的障碍。衰老的一个独特特征是许多可溶性因子的释放，即所谓的衰老相关分泌表型，它们在触发旁分泌衰老信号中发挥着关键作用。最近，有证据表明这种表型不仅包括可溶性因子，还包括EV。为了确定与 H-Ras 诱导的 EV 衰老相关的脂质特征，我们在人成纤维细胞中表达活性 H-Ras (H-RasV12)，并研究它如何影响 EV 释放和脂质组成。富含羟基化鞘磷脂、溶血和醚连接的磷脂以及特定的 H-Ras 诱导的衰老特征，例如与细胞相比，在 EV 中发现了鞘磷脂、溶血磷脂酸和硫苷脂。此外，成纤维细胞中的 H-RasV12 表达与参与囊泡形成的四跨膜蛋白水平较高有关。
Cells release extracellular vesicles (EVs) in their environment and cellular lipids play an important role in their formation, secretion and uptake. Besides, there is also evidence that EV transferred lipids impact on recipient’s cell signaling. Cellular senescence is characterized by a state of permanent proliferation arrest and represents a barrier towards the development of neoplastic lesions. A peculiar feature of senescence is the release of many soluble factors, the so-called Senescence-Associated Secretory Phenotype, which play a key role in triggering paracrine senescence signals. Recently, evidences have suggested that this phenotype includes not only soluble factors, but also EVs. To identify lipid signatures associated with H-Ras-induced senescence in EVs, we expressed active H-Ras (H-RasV12) in human fibroblasts and investigated how it affects EV release and lipid composition. An enrichment of hydroxylated sphingomyelin, lyso- and ether-linked phospholipids and specific H-Ras-induced senescence signatures, e.g. sphingomyelin, lysophosphatidic acid and sulfatides, were found in EVs compared to cells. Furthermore, H-RasV12 expression in fibroblasts was associated with higher levels of tetraspanins involved in vesicle formation.
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影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
影响因子：
3.5
作者：
Laulagnier, K;Grand, D;Record, M
通讯作者：
Record, M
影响因子：
64.8
作者：
Di Micco, Raffaella;Fumagalli, Marzia;di Fagagna, Fabrizio d'Adda
通讯作者：
di Fagagna, Fabrizio d'Adda
影响因子：
7.3
作者：
Andreu Z;Yáñez-Mó M
通讯作者：
Yáñez-Mó M
DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde