Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin.
Segregation and Heritability of Male Sterility in Populations Derived from Progeny of Satsuma Mandarin.
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DOI：
10.1371/journal.pone.0162408
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Shimizu T
中科院分区：
文献类型：
--
作者：
Goto S;Yoshioka T;Ohta S;Kita M;Hamada H;Shimizu T
日本利用温州蜜柑的雄性不育性培育无核柑橘品种，这对消费者来说是一种理想的性状。雄性不育通常通过花药发育或花粉育性来评价，然而，温州蜜柑雄性不育的遗传和遗传力知之甚少。本文研究了温州蜜柑雄性不育的遗传方式和广义遗传力。最初，我们评估了每个花药的花粉粒总数和明显的花粉育性，如乳酚蓝染色所示，在15个柑橘品种和选择，以了解温州蜜柑的雄性不育。结果表明，温州蜜柑雄性不育的主要原因是花药中花粉粒数减少。同时，对以温州蜜柑为亲本的3个F_1群体（'兴津56号' × '兴津46号' × '兴津56号'和'兴津46号' × '卡拉'）的上述性状进行了评价。在这些群体中的个体表现出强烈的分离，每花药的花粉粒数。花粉的表观育性也有分离现象，但基本稳定在70%~ 90%。在“冲津46号”ד冲津56号”和“冲津46号”ד卡拉”群体中，每花药花粉粒数的广义遗传力高达0.898。这些结果表明，温州蜜柑花粉粒数是决定其后代雄性不育性的主要因素，且该性状可遗传给后代。利用“兴津46号”ד兴津56号”和“兴津46号”ד卡拉”的F1群体，开发与雄性不育紧密连锁的DNA标记，有望为无核柑橘新品种的选育提供参考。
Male sterility derived from Satsuma mandarin (Citrus unshiu) has been used in Japanese citrus breeding programs to obtain seedless cultivars, which is a desirable trait for consumers. Male sterility has often been evaluated by anther development or pollen fertility; however, the inheritance and heritability of male sterility derived from Satsuma is poorly understood. In this study, we investigated the mode of inheritance and broad-sense heritability of male sterility derived from Satsuma. Initially, we evaluated the total number of pollen grains per anther and apparent pollen fertility, as indicated by lactophenol blue staining, in 15 citrus cultivars and selections to understand the male sterility of Satsuma. The results indicated that male sterility was primarily caused by decreased number of pollen grains per anther in progeny of Satsuma. We also evaluated these traits in three F1 populations (hyuganatsu × ‘Okitsu No. 56’, ‘Okitsu No. 46’ × ‘Okitsu No. 56’ and ‘Okitsu No. 46’ × ‘Kara’), of which the parents are derived from Satsuma. Individuals in these populations showed strong segregation for number of pollen grains per anther. The apparent fertility of pollen also showed segregation but was almost constant at 70%–90%. The estimated broad-sense heritability for the number of pollen grains per anther was as high as 0.898 in the ‘Okitsu No. 46’ × ‘Okitsu No. 56’ and ‘Okitsu No. 46’ × ‘Kara’ populations. These results indicated that the number of pollen grains per anther primarily determined male sterility among progeny of Satsuma, and this trait was inherited by the progeny. Development of DNA markers closely linked to male sterility using the F1 populations of ‘Okitsu No. 46’ × ‘Okitsu No. 56’ and ‘Okitsu No. 46’ × ‘Kara’ is expected to contribute to the breeding of novel seedless citrus cultivars.
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影响因子：
14.9
作者：
Kaminuma E;Fujisawa T;Tanizawa Y;Sakamoto N;Kurata N;Shimizu T;Nakamura Y
通讯作者：
Nakamura Y
影响因子：
3
作者：
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通讯作者：
Guo, Wen-Wu
影响因子：
1.9
作者：
Nishio, Sogo;Yamada, Masahiko;Saito, Toshihiro
通讯作者：
Saito, Toshihiro
影响因子：
1.9
作者：
Yamada, M;Sato, A;Ukai, Y
通讯作者：
Ukai, Y
影响因子：
2.4
作者：
Kunihisa M;Moriya S;Abe K;Okada K;Haji T;Hayashi T;Kim H;Nishitani C;Terakami S;Yamamoto T
通讯作者：
Yamamoto T