Human corneal limbal epithelial cell response to varying silk film geometric topography in vitro.
Human corneal limbal epithelial cell response to varying silk film geometric topography in vitro.
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DOI：
10.1016/j.actbio.2012.06.009
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发表时间：
2012-10
影响因子：
9.7
通讯作者：
Rosenblatt MI
中科院分区：
文献类型：
--
作者：
Lawrence BD;Pan Z;Liu A;Kaplan DL;Rosenblatt MI
Silk fibroin films are a promising class of biomaterials that have a number of advantages for use in ophthalmic applications due to their transparent nature, mechanical properties and minimal inflammatory response upon implantation. Freestanding silk films with parallel line and concentric ring topographies were generated for in vitro characterization of human corneal limbal-epithelial (HCLE) cell response upon differing geometric patterned surfaces. Results indicated that silk film topography significantly affected initial HCLE culture substrate attachment, cellular alignment, cell-to-cell contact formation, actin cytoskeleton alignment, and focal adhesion (FA) localization. Most notably, parallel line patterned surfaces displayed a 36%–54% increase on average in initial cell attachment, which corresponded to an over 2-fold increase in FA localization when compared to other silk film surfaces and controls. In addition, distinct localization of FA formation was observed along the edges for all patterned silk film topographies. In conclusion, silk film feature topography appears to help direct corneal epithelial cell response and cytoskeleton development, especially in regards to FA distribution, in vitro.
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影响因子：
14
作者：
Evans, MDM;McFarland, GA;Walboomers, XF
通讯作者：
Walboomers, XF
影响因子：
14
作者：
Lawrence BD;Marchant JK;Pindrus MA;Omenetto FG;Kaplan DL
通讯作者：
Kaplan DL
影响因子：
64.8
作者：
Jin, HJ;Kaplan, DL
通讯作者：
Kaplan, DL
影响因子：
3.7
作者：
DUNN, GA;HEATH, JP
通讯作者：
HEATH, JP
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan