Differential proteomics in dequeened honeybee colonies reveals lower viral load in hemolymph of fertile worker bees.

Differential proteomics in dequeened honeybee colonies reveals lower viral load in hemolymph of fertile worker bees.
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脱酮蜜蜂菌落中的差异蛋白质组学显示,肥沃工人蜜蜂血淋巴的病毒载量较低。

DOI:
10.1371/journal.pone.0020043
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发表时间:
2011
期刊:
影响因子:
3.7
通讯作者:
Verleyen P
Verleyen P
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Cardoen D;Ernst UR;Van Vaerenbergh M;Boerjan B;de Graaf DC;Wenseleers T;Schoofs L;Verleyen P

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在蜜蜂的真社会中,蜂王是成千上万只不育工蜂中唯一能生育的雌性,这一现象几个世纪以来一直吸引着科学家。引起工蜂卵巢抑制的近因,以及在殖民地中失去蜂王和育雏后引起工蜂卵巢激活的因素,在很大程度上仍然未知。为了揭示调控网络中的关键参与者,我们对卵巢完全激活与卵巢发育不全的工人的血淋巴谱进行了蛋白质组学比较。这项研究的一个意想不到的发现是年龄匹配的工人不育和小核糖核酸样病毒蛋白的富集之间的相关性。另一方面,有生育能力的工人显示免疫系统潜在成分的上调。病毒感染是否直接导致工人不育,或者是不育工人免疫系统较弱的结果,还有待研究。
The eusocial societies of honeybees, where the queen is the only fertile female among tens of thousands sterile worker bees, have intrigued scientists for centuries. The proximate factors, which cause the inhibition of worker bee ovaries, remain largely unknown; as are the factors which cause the activation of worker ovaries upon the loss of queen and brood in the colony. In an attempt to reveal key players in the regulatory network, we made a proteomic comparison of hemolymph profiles of workers with completely activated ovaries vs. rudimentary ovaries. An unexpected finding of this study is the correlation between age matched worker sterility and the enrichment of Picorna-like virus proteins. Fertile workers, on the other hand, show the upregulation of potential components of the immune system. It remains to be investigated whether viral infections contribute to worker sterility directly or are the result of a weaker immune system of sterile workers.
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