Macrophages and adipocytes in human obesity: adipose tissue gene expression and insulin sensitivity during calorie restriction and weight stabilization.
Macrophages and adipocytes in human obesity: adipose tissue gene expression and insulin sensitivity during calorie restriction and weight stabilization.
复制标题
作者：
Capel F;Klimcáková E;Viguerie N;Roussel B;Vítková M;Kováciková M;Polák J;Kovácová Z;Galitzky J;Maoret JJ;Hanácek J;Pers TH;Bouloumié A;Stich V;Langin D
我们研究了在饮食减肥计划的不同阶段脂肪组织基因表达的调节及其与胰岛素敏感性的关系。22名肥胖女性遵循饮食干预计划，包括4周极低热量饮食的能量限制阶段和2个月低热量饮食，随后3-4个月体重维持饮食的体重稳定期。在每个时间点，进行正常血糖-高胰岛素钳夹和皮下脂肪组织活检。脂肪组织基因表达谱进行了使用DNA微阵列在一个小组的8名妇女。采用RT-定量PCR方法检测22例脂肪组织巨噬细胞31种标志物的mRNA水平。在饮食干预计划期间，体重、脂肪量和C反应蛋白水平下降，葡萄糖处置率增加。转录组分析揭示了两种主要的变异模式。第一个涉及464个主要参与代谢的脂肪细胞基因，这些基因在能量限制期间下调，在体重稳定期间上调，在饮食干预期间不变。第二组包括511个主要参与炎症途径的巨噬细胞基因，这些基因在能量限制期间没有改变或上调，在体重稳定和饮食干预期间下调。因此，巨噬细胞标志物在能量限制期间上调，在体重稳定和饮食干预期间下调。每个饮食阶段葡萄糖处置率的增加与80-110个基因组的表达变化有关，这些基因在能量限制、体重稳定和饮食干预方面有所不同。脂肪组织巨噬细胞和脂肪细胞在饮食减肥计划的各个阶段显示出不同的基因调控模式和与胰岛素敏感性的关联。
We investigated the regulation of adipose tissue gene expression during different phases of a dietary weight loss program and its relation with insulin sensitivity. Twenty-two obese women followed a dietary intervention program composed of an energy restriction phase with a 4-week very-low-calorie diet and a weight stabilization period composed of a 2-month low-calorie diet followed by 3–4 months of a weight maintenance diet. At each time point, a euglycemic-hyperinsulinemic clamp and subcutaneous adipose tissue biopsies were performed. Adipose tissue gene expression profiling was performed using a DNA microarray in a subgroup of eight women. RT–quantitative PCR was used for determination of mRNA levels of 31 adipose tissue macrophage markers (n = 22). Body weight, fat mass, and C-reactive protein level decreased and glucose disposal rate increased during the dietary intervention program. Transcriptome profiling revealed two main patterns of variations. The first involved 464 mostly adipocyte genes involved in metabolism that were downregulated during energy restriction, upregulated during weight stabilization, and unchanged during the dietary intervention. The second comprised 511 mainly macrophage genes involved in inflammatory pathways that were not changed or upregulated during energy restriction and downregulated during weight stabilization and dietary intervention. Accordingly, macrophage markers were upregulated during energy restriction and downregulated during weight stabilization and dietary intervention. The increase in glucose disposal rates in each dietary phase was associated with variation in expression of sets of 80–110 genes that differed among energy restriction, weight stabilization, and dietary intervention. Adipose tissue macrophages and adipocytes show distinct patterns of gene regulation and association with insulin sensitivity during the various phases of a dietary weight loss program.
登录
查看更多内容
DOI：
10.1084/jem.157.4.1360
发表时间：
1983-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Pekala P;Kawakami M;Vine W;Lane MD;Cerami A
通讯作者：
Cerami A
影响因子：
4.8
作者：
Clément, K;Viguerie, N;Langin, D
通讯作者：
Langin, D
影响因子：
37.8
作者：
Miranville, A;Heeschen, C;Bouloumié, A
通讯作者：
Bouloumié, A
DOI：
10.1073/pnas.79.3.912
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KAWAKAMI, M;PEKALA, PH;CERAMI, A
通讯作者：
CERAMI, A
爱丁堡人类代谢网络重建及其功能分析。
影响因子：
9.9
作者：
Ma, Hongwu;Sorokin, Anatoly;Mazein, Alexander;Selkov, Alex;Selkov, Evgeni;Demin, Oleg;Goryanin, Igor
通讯作者：
Goryanin, Igor