Breaking the loop: Tackling homoacetogenesis by chloroform to halt hydrogen production-consumption loop in single chamber microbial electrolysis cells
Breaking the loop: Tackling homoacetogenesis by chloroform to halt hydrogen production-consumption loop in single chamber microbial electrolysis cells
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打破循环：通过氯仿解决同型乙酸生成问题，以终止单室微生物电解池中的氢气生产-消耗循环
DOI：
10.1016/j.cej.2020.124436
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发表时间：
2020-06
影响因子：
15.1
通讯作者：
Liu Hong
中科院分区：
文献类型：
--
作者：
Wang Luguang;Chen Ye;Long Fei;Singh Lakhveer;Trujillo Stephanie;Xiao Xiang;Liu Hong
在单室微生物电解池（MECs）中，均质产氢和产氢-耗氢循环的存在严重阻碍了氢气的有效回收。然而，迄今为止还没有提出有效的抑制同质醋酸菌的方法。在这项研究中，我们证明了氯仿在mec中作为同质醋酸原抑制剂的有效性。在小型mec （320 mL）间歇式运行中，浓度为0.02% （v/v）和0.03% （v/v）的氯仿停止了与不可发酵底物（醋酸酯）的均质丙酮生成，将氢气产率和阴极氢回收率分别从21%和14%提高到94%和90%。当氯仿浓度从0% （v/v）增加到0.03% （v/v）时，电流密度（基于阳极表面积）仅从18 A/m2下降到13 A/m2，对电化学性能没有显著影响。然而，当使用可发酵底物（葡萄糖）时，0.01% （v/v）氯仿对同质醋酸菌的抑制作用导致电流密度显著降低，这可能是由于乙酸的产生减少，从而降低了用于产电的底物浓度。然而，在较大的MEC （10 L）中，使用固定化污泥培养和较高的葡萄糖浓度（56 mM）， 0.02% （v/v）氯仿将产氢率从0 L/L/D提高到4.9 L/L/D，电流密度从12-16 a /m2提高到18-21 a /m2。微生物群落分析表明，同质产醋酸菌属。在阴极生物膜和浮游细胞中被氯仿清除。未来通过降低氯仿残留浓度和开发更环保的机制相似的抑制剂对该方法进行改进，可能会在mec中得到实际应用。
The presence of homoacetogenesis and hydrogen production-consumption loop significantly hinders the efficient hydrogen recovery in single chamber microbial electrolysis cells (MECs). However, no effective inhibition method has been proposed against homoacetogens hitherto. In this study, we demonstrated the effectiveness of chloroform as a homoacetogen inhibitor in MECs. In small MECs (320 mL) operated in batch mode, chloroform with concentrations of 0.02% (v/v) and 0.03% (v/v) ceased homoacetogenesis with non-fermentable substrate (acetate), enhancing hydrogen yield and cathodic hydrogen recovery from 21% and 14% to 94% and 90%, respectively. Electrochemical performance was not significantly affected as current density (based on anode surface area) only decreased from 18 A/m2to 13 A/m2with chloroform concentration increased from 0% (v/v) to 0.03% (v/v). However, when using a fermentable substrate (glucose), the inhibition against homoacetogens by 0.01% (v/v) chloroform led to a significant decrease in current density possibly due to the decreased production of acetate, which reduced the substrate concentration for exoelectrogenesis. Nevertheless, in a larger MEC (10 L), using an immobilized sludge culture and higher glucose concentration (56 mM), 0.02% (v/v) chloroform enhanced hydrogen production rate from 0 L/L/D to 4.9 L/L/D and current density from 12-16 A/m2to 18–21 A/m2. Microbial community analysis revealed that the homoacetogenicAcetobacteriumspp. was eliminated in the cathodic biofilms and planktonic cells by chloroform. Future improvement of this method, through reducing the residual chloroform concentration and developing more environment-friendly inhibitor using similar mechanisms, may lead to the practical application in MECs.
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DOI：
10.1016/j.cep.2018.04.006
发表时间：
2018-06-01
影响因子：
4.3
作者：
Sepehri, Arsalan;Sarrafzadeh, Mohammad-Hossein
通讯作者：
Sarrafzadeh, Mohammad-Hossein
影响因子：
11.4
作者：
Luguang Wang;S. Trujillo;Hong Liu
通讯作者：
Luguang Wang;S. Trujillo;Hong Liu
共养相互作用驱动微生物电解池中低温葡萄糖产氢
影响因子：
11.4
作者：
Lu, Lu;Xing, Defeng;Logan, Bruce E.
通讯作者：
Logan, Bruce E.
影响因子：
7.2
作者：
Hu, Bo;Chen, Shulin
通讯作者：
Chen, Shulin
影响因子：
4
作者：
Xu, S.;Liu, H.
通讯作者：
Liu, H.