Electrospun conductive nanofiber yarns for accelerating mesenchymal stem cells differentiation and maturation into Schwann cell-like cells under a combination of electrical stimulation and chemical induction.
Electrospun conductive nanofiber yarns for accelerating mesenchymal stem cells differentiation and maturation into Schwann cell-like cells under a combination of electrical stimulation and chemical induction.
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DOI：
10.1016/j.actbio.2020.11.042
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发表时间：
2022-03
影响因子：
9.7
通讯作者：
Duan B
中科院分区：
文献类型：
--
作者：
Wu S;Qi Y;Shi W;Kuss M;Chen S;Duan B
在长间隙周围神经损伤的修复中，需要开发多功能管填充材料来改善现有神经引导导管的性能。本研究利用改进的静电纺丝装置制备了基于聚对二氧环酮（PPDO）生物聚合物和不同浓度碳纳米管的复合纳米纤维纱线（NYs）。我们证实了成功地将碳纳米管掺入到制备的复合NYs的PPDO纳米纤维中。与纯PPDO NYs相比，PPDO/CNT NYs具有相似的形态和结构。然而，与PPDO NYs相比，PPDO/CNT NYs的机械性能和电导率明显提高。生物学试验显示，添加CNTs对兔雪旺细胞（rSCs）的生长和增殖无负面影响，但能较好地维持rSCs的表型。我们还证明，电刺激（ES）显著增强了PPDO/CNT NYs上人脂肪源性间充质干细胞（hADMSCs）向sc样细胞（SCLCs）的分化能力。更重要的是，我们发现ES和化学诱导的独特组合通过显著上调SC髓鞘相关基因标记物的表达水平和增加生长因子的分泌，进一步增强了PPDO/CNT NYs上hadmsc - sclc的成熟。总的来说，本研究表明，我们的导电PPDO/CNT复合NYs可以为各种细胞活动提供有益的微环境，使其成为具有吸引力的ngc填充底物，用于PN再生应用。
Development of multifunctional tube-filling materials is required to improve the performances of the existing nerve guidance conduits (NGCs) in the repair of long-gap peripheral nerve (PN) injuries. In this study, composite nanofiber yarns (NYs) based on poly(p-dioxanone) (PPDO) biopolymer and different concentrations of carbon nanotubes (CNTs) were manufactured by utilizing a modified electrospinning apparatus. We confirmed the successful incorporation of CNTs into the PPDO nanofibers of as-fabricated composite NYs. The PPDO/CNT NYs exhibited similar morphology and structure in comparison with pure PPDO NYs. However, the PPDO/CNT NYs showed obviously enhanced mechanical properties and electrical conductivity compared to PPDO NYs. The biological tests revealed that the addition of CNTs had no negative effects on the cell growth, and proliferation of rabbit Schwann cells (rSCs), but it better maintained the phenotype of rSCs. We also demonstrated that the electrical stimulation (ES) significantly enhanced the differentiation capability of human adipose-derived mesenchymal stem cells (hADMSCs) into SC-like cells (SCLCs) on the PPDO/CNT NYs. More importantly, a unique combination of ES and chemical induction was found to further enhance the maturation of hADMSC-SCLCs on the PPDO/CNT NYs by notably upregulating the expression levels of SC myelination-associated gene markers and increasing the growth factor secretion. Overall, this study showed that our electrically conductive PPDO/CNT composite NYs could provide a beneficial microenvironment for various cell activities, making them an attractive candidate as NGC-infilling substrates for PN regeneration applications.
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影响因子：
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作者：
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发表时间：
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影响因子：
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