PtrMYB3, a R2R3-MYB Transcription Factor from Poncirus trifoliata, Negatively Regulates Salt Tolerance and Hydrogen Peroxide Scavenging.
PtrMYB3, a R2R3-MYB Transcription Factor from Poncirus trifoliata, Negatively Regulates Salt Tolerance and Hydrogen Peroxide Scavenging.
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PtrMYB3 是一种来自枳壳的 R2R3-MYB 转录因子，可负调节盐耐受性和过氧化氢清除能力
DOI：
10.3390/antiox10091388
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发表时间：
2021-08-30
期刊：
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Wei T;Guo D;Liu J
MYB转录因子广泛存在于植物中，在非生物胁迫中发挥重要作用。然而，大多数MYB基因尚未在植物中被鉴定，其在非生物胁迫中的功能仍然未知。本研究从橙子（Poncirus trifoliata（L.）Raf.），并对其在植物耐盐性中的作用进行了研究。PtrMYB 3含有一个保守的R2 R3-MYB结构域，这是R2 R3-MYB亚家族蛋白的典型特性。非生物胁迫下的表达谱分析表明，PtrMYB 3可以诱导盐，脱水和冷胁迫。PtrMYB 3定位于细胞核，具有反式激活活性。在烟草中通过遗传转化过表达PtrMYB 3降低了烟草的耐盐性，而在枳橙子中通过VIGS（virus-induced gene silencing）沉默PtrMYB 3可显著增强烟草的耐盐性，表明PtrMYB 3负调控烟草的耐盐性。此外，过氧化物酶基因（PtrPOD）被发现在PtrMYB 3沉默的枳橙子中被极大地上调，并且双重LUC（荧光素酶）测定证实PtrMYB 3可以抑制PtrPOD的表达。盐胁迫后，转PtrMYB 3基因烟草中H2 O2的积累量高于野生型烟草，进一步证实了PtrMYB 3的过表达抑制了PtrPOD介导的H2 O2清除。综上所述，这些结果表明，PtrMYB 3负调节耐盐性，至少部分，由于过量积累的H2 O2。
MYB transcription factors are widely present in plants and play significant roles in abiotic stresses. However, most MYB genes have not been identified in plants and their functions in abiotic stresses are still unknown. In this study, one MYB gene, designated as PtrMYB3, was cloned from trifoliate orange (Poncirus trifoliata (L.) Raf.), and its function in salt tolerance was investigated. PtrMYB3 contains a conserved R2R3-MYB domain, which is the typical property of R2R3-MYB subfamily proteins. Expression profiling under abiotic stresses indicated that PtrMYB3 could be induced by salt, dehydration and cold stresses. PtrMYB3 was found to be localized to the nucleus and possessed transactivation activity. Overexpression of PtrMYB3 by genetic transformation in tobacco impaired its salt tolerance, whereas silencing of PtrMYB3 by VIGS (virus-induced gene silencing) in trifoliate orange conferred significantly enhanced salt tolerance, indicating that PtrMYB3 negatively regulates salt tolerance. Furthermore, a peroxidase gene (PtrPOD) was found to be greatly upregulated in PtrMYB3-silenced trifoliate orange, and a dual LUC (luciferase) assay confirmed that PtrMYB3 could suppress the expression of PtrPOD. The hydrogen peroxide (H2O2) accumulation in PtrMYB3 transgenic tobacco plants after salt stress was higher than the wild type (WT), further confirming that overexpression of PtrMYB3 inhibited PtrPOD-mediated H2O2 scavenging. Taken together, these results demonstrate that PtrMYB3 negatively regulates salt tolerance, at least in part, due to the excess accumulation of H2O2.
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