Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation.
Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation.
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DOI：
10.1007/s12015-018-9854-5
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发表时间：
2019-03
影响因子：
4.8
通讯作者：
Huang GT
中科院分区：
文献类型：
--
作者：
Li D;Zou XY;El-Ayachi I;Romero LO;Yu Z;Iglesias-Linares A;Cordero-Morales JF;Huang GT
包括口腔干细胞(OSCs)在内的人骨髓间充质干细胞(MSCs)作为细胞来源分化为功能性神经元的潜力尚不确定。在这里，我们测试了一些人类OSCs与非OSCs的神经源性潜能，并采用了各种神经源性诱导方法。OSCs包括牙髓干细胞(DPSCs)、牙周间充质干细胞(GMSCs)、根尖乳头干细胞和非OSCs包括骨髓MSCs(BMMSCs)、包皮成纤维细胞和真皮成纤维细胞。成熟末期细胞进行RT-qPCR、免疫细胞荧光检测神经源性基因表达或电生理分析。我们发现，与其他细胞相比，诱导的DPSCs和GMSCs总体上似乎更具神经源性，无论是在形态上还是在神经源性基因表达的水平上。尽管如此，在所有采用的神经诱导方法中，只有一种由神经球介导的方法产生了功能神经元的电生理特性。在这种方法下，细胞在分化的第一阶段表达增加的神经干细胞标记物Nestin和SOX1。βⅢ-微管蛋白、CNPase、胶质纤维酸性蛋白、MAP-2、NFM、PAN-NAV、GAD67、NAV1.6、NF1、NSE、PSD95和突触素在分化至成熟的第二阶段表达。电生理实验显示，8.3%的DPSC来源的神经细胞和21.2%的GMSC来源的神经细胞。
The potential of human mesenchymal stromal/stem cells (MSCs) including oral stem cells (OSCs) as a cell source to derive functional neurons has been inconclusive. Here we tested a number of human OSCs for their neurogenic potential compared to non-OSCs and employed various neurogenic induction methods. OSCs including dental pulp stem cells (DPSCs), gingiva-derived mesenchymal stem cells (GMSCs), stem cells from apical papilla and non-OSCs including bone marrow MSCs (BMMSCs), foreskin fibroblasts and dermal fibroblasts using non-neurosphere- mediated or neurosphere-mediated methods to guide them toward neuronal lineages. Cells were subjected to RT- qPCR, immunocytofluorescence to detect the expression of neurogenic genes or electrophysiological analysis at final stage of maturation. We found that induced DPSCs and GMSCs overall appeared to be more neurogenic compared to other cells either morphologically or levels of neurogenic gene expression. Nonetheless, of all the neural induction methods employed, only one neurosphere-mediated method yielded electrophysiological properties of functional neurons. Under this method, cells expressed increased neural stem cell markers, nestin and SOX1, in the first phase of differentiation. Neuronal-like cells expressed βIII-tubulin, CNPase, GFAP, MAP-2, NFM, pan-Nav, GAD67, Nav1.6, NF1, NSE, PSD95, and synapsin after the second phase of differentiation to maturity. Electrophysiological experiments revealed that 8.3% of DPSC-derived neuronal cells and 21.2% of GMSC-derived neuronal cells.
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影响因子：
7.5
作者：
Djouad F;Jackson WM;Bobick BE;Janjanin S;Song Y;Huang GT;Tuan RS
通讯作者：
Tuan RS
DOI：
10.1007/978-1-62703-128-8_30
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Louis, Sharon A;Mak, Carmen K H;Reynolds, Brent A
通讯作者：
Reynolds, Brent A
影响因子：
4.2
作者：
Huang, George T-J.;Shagramanova, Kristina;Chan, Selina W.
通讯作者：
Chan, Selina W.
影响因子：
5.2
作者：
Cho, KJ;Trzaska, KA;Rameshwar, P
通讯作者：
Rameshwar, P
影响因子：
23.9
作者：
Marro, Samuele;Pang, Zhiping P.;Yang, Nan;Tsai, Miao-Chih;Qu, Kun;Chang, Howard Y.;Suedhof, Thomas C.;Wernig, Marius
通讯作者：
Wernig, Marius