Pulmonary fibrosis induced by H5N1 viral infection in mice.
Pulmonary fibrosis induced by H5N1 viral infection in mice.
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DOI：
10.1186/1465-9921-10-107
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发表时间：
2009-11-12
影响因子：
5.8
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Qiao J;Zhang M;Bi J;Wang X;Deng G;He G;Luan Z;Lv N;Xu T;Zhao L
炎症过程导致肺损伤，可能导致肺纤维化（PF）。在这里，我们描述了PF感染H5 N1病毒的小鼠。8周龄BALB/c小鼠鼻内接种1 × 101 MID 50的A/Chicken/Hebei/108/2002（H5 N1）病毒。通过观察接种后第7、14和30天的羟脯氨酸浓度、肺指数和组织病理学来评价肺损伤/纤维化。接种H5 N1病毒的小鼠在感染后30天内出现了两个阶段的肺部疾病。急性期，感染小鼠于接种后第7天出现典型的弥漫性肺炎，伴有炎性细胞浸润、肺泡和间质水肿和出血。在恢复期，大多数感染小鼠在接种后30天发生不同程度的PF，18%的存活小鼠发生严重的肺泡间质和肺泡内纤维化，肺泡壁增厚，肺泡塌陷和大面积纤维化。H5 N1感染小鼠的羟脯氨酸水平显著升高，表明肺中胶原沉积，并证实肺纤维化。H5 N1感染组小鼠的肺干重/体重比显著增加，这可能与H5 N1感染小鼠肺纤维化的形成有关。我们的研究结果表明，H5 N1感染的小鼠在恢复期出现了典型的PF，这将有助于研究人类H5 N1疾病的纤维化和潜在的治疗干预。
Inflammatory process results in lung injury that may lead to pulmonary fibrosis (PF). Here, we described PF in mice infected with H5N1 virus. Eight-week-old BALB/c mice were inoculated intranasally with 1 × 101 MID50 of A/Chicken/Hebei/108/2002(H5N1) viruses. Lung injury/fibrosis was evaluated by observation of hydroxyproline concentrations, lung indexes, and histopathology on days 7, 14, and 30 postinoculation. H5N1-inoculated mice presented two stages of pulmonary disease over a 30-d period after infection. At acute stage, infected-mice showed typical diffuse pneumonia with inflammatory cellular infiltration, alveolar and interstitial edema and hemorrhage on day 7 postinoculation. At restoration stage, most infected-mice developed PF of different severities on day 30 postinoculation, and 18% of the survived mice underwent severe interstitial and intra-alveolar fibrosis with thickened alveolar walls, collapsed alveoli and large fibrotic areas. The dramatically elevated hydroxyproline levels in H5N1-infected mice showed deposition of collagen in lungs, and confirmed fibrosis of lungs. The dry lung-to-body weight ratio was significantly increased in infected group, which might be associated with the formation of PF in H5N1-infected mice. Our findings show that H5N1-infected mice develop the typical PF during restoration period, which will contribute to the investigation of fibrogenesis and potential therapeutic intervention in human H5N1 disease.
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影响因子：
3.9
作者：
WOESSNER, JF
通讯作者：
WOESSNER, JF
禽-人流感流行模型
影响因子：
4.3
作者：
Iwami, Shingo;Takeuchi, Yasuhiro;Liu, Xianning
通讯作者：
Liu, Xianning
影响因子：
168.9
作者：
Claas, ECJ;Osterhaus, ADME;Webster, RG
通讯作者：
Webster, RG
影响因子：
3.2
作者：
Ouchi, Hiroshi;Fujita, Masaki;Nakanishi, Yoichi
通讯作者：
Nakanishi, Yoichi
影响因子：
5.8
作者：
Allen JT;Spiteri MA
通讯作者：
Spiteri MA