Differential Inhibition of Sox10 Functions by Notch-Hes Pathway
Differential Inhibition of Sox10 Functions by Notch-Hes Pathway
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Notch-Hes 通路对 Sox10 功能的差异抑制
DOI：
10.1007/s10571-019-00764-7
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发表时间：
2019-11
影响因子：
4
通讯作者：
Qiu Mengsheng
中科院分区：
文献类型：
--
作者：
Xiao Guanxiu;Du Junqing;Wu Huihui;Ge Xinqi;Xu Xiaofeng;Yang Aifen;Zhu Ying;Hu Xuemei;Zheng Kang;Zhu Qiang;Qiu Mengsheng
在发育中的中枢神经系统中，少突胶质细胞（oligodendrocytes，OLs）的终末分化受到内外因素的共同调控。最近的研究表明，Notch-Hes信号通路影响培养和发育过程中少突胶质细胞的成熟。然而，参与少突胶质细胞成熟的特异性Notch受体及其下游效应子Hes基因尚未得到系统研究。在这项研究中，我们发现，Notch 1和Notch 3表达在少突胶质细胞前体细胞（OPC）在胶质细胞生成过程中，和Hes 5是主要的Notch下游转录因子，是瞬时表达的OPC。Notch胞内结构域（NICD）和Hes 5蛋白在鸡胚脊髓中的过表达抑制了内源性和Sox 10诱导的Mbp基因表达。出乎意料的是，NICD/Hes 5的过表达并没有抑制Sox 10诱导Olig 2表达和Myrf诱导Mbp表达，这表明NICD/Hes 5信号传导对髓鞘相关基因和早期祖基因的Sox 10激活的差异抑制作用。
In the developing central nervous system, the terminal differentiation of oligodendrocytes (OLs) is regulated by both extrinsic and intrinsic factors. Recent studies have suggested that the Notch-Hes signaling pathway influences the maturation of oligodendrocytes in culture and during development. However, the specific Notch receptors and their downstream effectors Hes genes that are involved in oligodendrocyte maturation have not been investigated systematically. In this study, we showed that Notch1 and Notch3 are expressed in oligodendrocyte precursor cells (OPCs) during gliogenesis, and Hes5 is the major Notch downstream transcription factor that is transiently expressed in OPCs. Overexpression of Notch intracellular domain (NICD) and Hes5 proteins in embryonic chicken spinal cord suppressed both the endogenous and Sox10-induced Mbp gene expression. Unexpectedly, overexpression of NICD/Hes5 did not inhibit Sox10 induction of Olig2 expression and Myrf induced Mbp expression, suggesting the differential inhibitory effects of NICD/Hes5 signaling on Sox10 activation of myelin-related genes and early progenitor genes.
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影响因子：
5.6
作者：
He L;Lu QR
通讯作者：
Lu QR
影响因子：
10.5
作者：
Stolt, CC;Rehberg, S;Wegner, M
通讯作者：
Wegner, M
NOTCH1控制脊髓中的少突胶质细胞分化。
影响因子：
7.8
作者：
Genoud, Stephane;Lappe-Siefke, Corinna;Goebbels, Sandra;Radtke, Freddy;Aguet, Michel;Scherer, Steven S;Suter, Ueli;Nave, Klaus-Armin;Mantei, Ned
通讯作者：
Mantei, Ned
影响因子：
16.2
作者：
Zhou, Q;Wang, SL;Anderson, DJ
通讯作者：
Anderson, DJ
影响因子：
4.6
作者：
T. Kondo;M. Raff
通讯作者：
T. Kondo;M. Raff