The effects of opioid receptor antagonists suggest that testicular opiates regulate Sertoli and Leydig cell function in the neonatal rat.
The effects of opioid receptor antagonists suggest that testicular opiates regulate Sertoli and Leydig cell function in the neonatal rat.
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阿片受体拮抗剂的作用表明睾丸阿片类药物调节新生大鼠的支持细胞和间质细胞功能。
DOI：
10.1210/endo-118-5-2039
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发表时间：
1986
期刊：
影响因子：
4.8
通讯作者：
C. Bardin
中科院分区：
文献类型：
--
作者：
I. Gerendai;C. Shaha;G. Gunsalus;C. Bardin
β-内啡肽和其他来源于前阿片黑素皮质素的多肽在睾丸间质细胞中合成。为了更好地了解这些阿片肽和其他内源性阿片肽在睾丸中的可能功能，阿片类拮抗剂纳洛酮和纳美芬被注射到5天龄的半衰期大鼠的睾丸内。纳洛酮和纳美芬均可促进11日龄大鼠单侧睾丸切除所致的睾丸肥大。出乎意料的是，产生最大肥厚所需的纳美芬剂量至少比之前报道的纳洛酮低100倍。通过测定基础睾酮产量和血清雄激素结合蛋白(RABP)来评价睾丸间质细胞和支持细胞的功能。纳洛酮的最佳肥大剂量(1微克/睾丸)抑制了睾酮的产生，并对rABP的分泌产生了不均匀的影响(要么没有影响，要么略有增加)。相比之下，治疗肥大的最佳纳美芬剂量(0.01微克/睾丸)不仅抑制了基础睾酮的分泌，而且均匀地提高了血清中rABP水平。较大剂量的阿片类拮抗剂，高达1微克/睾丸，对所测量的三个参数(肥大、睾酮分泌和rABP水平)没有同样的效果。这些结果表明，该药在睾丸中既有拮抗作用，又有兴奋作用。在对肥厚产生最佳效果的剂量下，全身给药这些拮抗剂没有效果。这些研究结果表明，睾丸内阿片类药物对支持细胞的生长和rABP的分泌具有抑制作用。此外，这些多肽还可能调节间质细胞的睾酮分泌。
beta-Endorphin and other peptides derived from proopiomelanocortin are synthesized in testicular Leydig cells. To better understand the possible function of these and other endogenous opioid peptides in the testis, the opioid antagonists naloxone and nalmefene were administered intratesticularly to hemicastrated 5-day-old rats. Both naloxone and nalmefene potentiated testicular hypertrophy induced by unilateral orchidectomy at 11 days of age. Unexpectedly, at least a 100-fold lower dose of nalmefene was required to produce maximal hypertrophy than that previously reported for naloxone. Leydig and Sertoli cell functions were evaluated, respectively, by measurement of basal testosterone production in vitro and rat androgen-binding protein (rABP) in serum. The optimal dose of naloxone for hypertrophy (1 microgram/testis) suppressed testosterone production and had a nonuniform effect on rABP secretion (either had no effect or produced a slight increase). By contrast, the optimal dose of nalmefene for hypertrophy (0.01 microgram/testis) not only suppressed basal testosterone secretion, but also uniformly increased rABP levels in serum. Larger doses of this opioid antagonist, up to 1 microgram/testis, were not as effective on the three parameters measured (hypertrophy, testosterone secretion, and rABP levels). These results suggest that this agent has both antagonistic and agonistic activities in the testis. At the doses that produced optimal effects on hypertrophy, systemic administration of these antagonists produced no effects. The results of these studies suggest that intratesticular opiates exert a suppressive effect on Sertoli cell growth and rABP secretion. In addition, these peptides may modulate testosterone secretion by Leydig cells.
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β-内啡肽存在于五个物种的雄性生殖道中。
影响因子：
3.6
作者：
Shu-Dong,T;Phillips,DM;Halmi,N;Krieger,D;Bardin,CW
通讯作者：
Bardin,CW
成年大鼠睾丸中阿黑皮质素原信使 RNA 的表征和定位。
DOI：
10.1126/science.6740329
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Pintar,JE;Schachter,BS;Herman,AB;Durgerian,S;Krieger,DT
通讯作者：
Krieger,DT
半去势会导致未成熟大鼠睾丸中支持细胞对 [3H] 胸苷的增强摄取，而睾酮会阻止其增强。
影响因子：
3.6
作者：
Orth,JM;Higginbotham,CA;Salisbury,RL
通讯作者：
Salisbury,RL
阿片黑皮质素原样基因在睾丸和附睾中的表达。
DOI：
10.1073/pnas.81.18.5672
发表时间：
1984
影响因子：
11.1
作者：
Chen,CL;Mather,JP;Morris,PL;Bardin,CW
通讯作者：
Bardin,CW