Hemicastration causes and testosterone prevents enhanced uptake of [3H] thymidine by Sertoli cells in testes of immature rats.
Hemicastration causes and testosterone prevents enhanced uptake of [3H] thymidine by Sertoli cells in testes of immature rats.
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半去势会导致未成熟大鼠睾丸中支持细胞对 [3H] 胸苷的增强摄取，而睾酮会阻止其增强。
DOI：
10.1095/biolreprod30.1.263
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发表时间：
1984
影响因子：
3.6
通讯作者：
Salisbury,RL
中科院分区：
文献类型：
--
作者：
Orth,JM;Higginbotham,CA;Salisbury,RL
将大鼠幼仔进行半去势，并在手术后8小时至21天的时间间隔内将剩余睾丸中支持细胞对[3H]胸苷的摄取与假手术幼仔的睾丸中的摄取进行比较。处死前2小时皮下注射标记的胸苷。对睾丸进行光学显微镜放射自显影，并通过将组织切片中的细胞核标记为标记或未标记来确定掺入[3H]胸苷的支持细胞核的百分比。手术后 8 小时，半去势动物中标记的细胞百分比比完整对照有所增加，并且第二天半去势动物中睾丸肥大变得明显。半阉割中支持细胞的标记持续升高 4 天，然后恢复正常。手术后 4 天测量两组血浆促性腺激素水平时，发现半去势者的卵泡刺激素 (FSH) 是正常值的两倍多，而黄体生成素 (LH) 则没有变化。还检查了睾酮对支持细胞对半去势反应的影响。在半阉割者中，2天的雄激素治疗抑制了支持细胞的增殖反应，另外2天则取消了支持细胞的增殖反应。我们的研究结果表明，剩余睾丸内支持细胞增殖的增加与半去势后睾丸的增大有关。他们还暗示，睾酮预防代偿性肥大涉及以某种方式干扰支持细胞的这种反应。最后，我们的数据表明 FSH 控制未成熟大鼠体内支持细胞的增殖。
Rat pups were hemicastrated and uptake of [3H] thymidine by Sertoli cells in the remaining testis was compared to that in testes of sham-operated pups at intervals of from 8 h to 21 days after surgery. Labeled thymidine was administered subcutaneously 2 h before sacrifice. Testes were processed for light microscope autoradiography and the percent of Sertoli cell nuclei that had incorporated [3H] thymidine was determined by scoring nuclei in tissue sections as labeled or unlabeled. The percentage of cells labeled was increased in hemicastrates over intact controls by 8 h after surgery and testicular hypertrophy became apparent in hemicastrates by the following day. Labeling of Sertoli cells in hemicastrates remained elevated for 4 days and then returned to normal. When plasma levels of gonadotropins were measured in both groups 4 days after surgery, follicle-stimulating hormone (FSH) was found to be more than twice normal in hemicastrates while luteinizing hormone (LH) was unchanged. The effect of testosterone on the response of Sertoli cells to hemicastration was also examined. In hemicastrates, 2 days of androgen therapy depressed, and an additional 2 days abolished, the proliferative response of the Sertoli cells. Our findings suggest that increased proliferation of Sertoli cells within the remaining testis is involved in the enlargement of the testis that follows hemicastration. They also imply that prevention of compensatory hypertrophy by testosterone involves interference with this response of Sertoli cells in some way. Finally, our data implicate FSH in control of Sertoli cell proliferation in vivo in immature rats.
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血浆放射免疫测定揭示了大鼠垂体 FSH 对单侧性腺切除反应的性别差异。
影响因子：
4.8
作者：
V. Ramirez;C. Sawyer
通讯作者：
C. Sawyer
单侧睾丸切除术对大鼠睾丸的影响。
DOI：
--
发表时间：
1956
期刊：
影响因子：
--
作者：
Grant Jh
通讯作者：
Grant Jh
大鼠睾丸肥大。
DOI：
10.1530/jrf.0.0210537
发表时间：
1970
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
D. S. Liang;M. D. Liang
通讯作者：
M. D. Liang
影响因子：
4.8
作者：
OJEDA, SR;RAMIREZ, VD
通讯作者：
RAMIREZ, VD
影响因子：
3.6
作者：
SOLARI, AJ;FRITZ, IB
通讯作者：
FRITZ, IB