Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant
Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant
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基因工程花青素途径可提高茄子中促进健康色素的产量
DOI：
10.1007/s11032-016-0454-2
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发表时间：
2016-04
影响因子：
3.1
通讯作者：
Chen Guoping
中科院分区：
文献类型：
--
作者：
Zhang Yanjie;Chu Guihua;Hu Zongli;Gao Qiong;Cui Baolu;Tian Shibing;Wang Bo;Chen Guoping
花青素是广泛分布于植物界的具有高抗氧化活性的天然色素，在各种生理过程中起着重要作用。由于这些促进健康的色素具有醒目的颜色和特殊的营养成分，人们一直致力于提高蔬菜和谷物中花青素的含量。此前，我们报道了编码R2R3 MYB转录因子的smmyb1基因参与了茄子果皮花青素生物合成的调控。在这项工作中，我们通过农杆菌介导的转化将smmyb1引入到一个不积累花青素的茄子品种（Solanum aethiopicumgroupGilo）中。在正常生长条件下，转基因植物的叶片、花瓣、雄蕊和果皮中花青素含量较高，尤其是果肉中。从紫果肉中分离出高度甲基化的花色素苷，分别为malvidin 3-（对香豆醇基）鼠李糖苷（葡萄糖苷）-5-葡萄糖苷和malvidin 3-（阿魏酰）鼠李糖苷（葡萄糖苷）-5-葡萄糖苷，并采用HPLC-ESI-MS /MS进行鉴定。qRT-PCR分析显示，与未转化植株相比，转基因植株组织中大部分花青素结构基因显著上调。此外，转基因幼苗对冰冻胁迫的耐受性更强，在复温条件下恢复能力更好。这些结果为今后选育具有更健康农艺性状的茄子新品种奠定了良好的基础。
Anthocyanins, natural pigments with high antioxidant activities, are widely distributed in the plant kingdom and play important roles in various physiological processes. Much effort has been committed to enhancing the anthocyanin content of these health-promoting pigments in vegetables and grains, for their eye-catching colors and special nutrients. Previously, we reported that theSmMYB1gene encoding a R2R3 MYB transcription factor participated in the regulation of anthocyanin biosynthesis in the peel of eggplant. In this work, we introducedSmMYB1into a non-anthocyanin-accumulating eggplant cultivar (Solanum aethiopicumgroupGilo) via Agrobacterium-mediated transformation. Genetically engineered plants exhibited high concentrations of anthocyanin in leaves, petals, stamens, and fruit peels under normal growth conditions, especially in fruit flesh. Furthermore, highly methylated anthocyanins, malvidin 3-(p-coumaroyl)rhamnoside(glucoside)-5-glucoside and malvidin 3-(feruloyl)rhamnoside(glucoside)-5-glucoside, were separated from the purple fruit flesh and identified by HPLC–ESI–MS/MS. qRT-PCR analysis revealed that most anthocyanin structural genes were dramatically up-regulated in the tissues of transgenic lines compared with non-transformed plants. In addition, the transgenic seedlings had greater tolerance to freezing stress and better recovery under rewarming conditions. These results provide a good foundation for the breeding of new eggplant cultivars with more healthy agronomic traits in future studies.
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影响因子：
2.2
作者：
R. Yakimchuk;J. Hoddinott
通讯作者：
R. Yakimchuk;J. Hoddinott
影响因子：
7.4
作者：
Winkel-Shirley, B
通讯作者：
Winkel-Shirley, B
影响因子：
3.3
作者：
Klaper, R;Frankel, S;Berenbaum, MR
通讯作者：
Berenbaum, MR
影响因子：
7.7
作者：
de Pascual-Teresa, Sonia;Sanchez-Ballesta, Maria Teresa
通讯作者：
Sanchez-Ballesta, Maria Teresa
影响因子：
6.2
作者：
Edgardo Filippone;P. Lurquin
通讯作者：
Edgardo Filippone;P. Lurquin